Although induction of contact skin sensitivity by low molecular weight 2,4-dinitrobenzenes requires the formation in vivo of 2,4-dinitrophenyl-proteins, analogous protein conjugates prepared in vitro are unable to induce this hypersensitive state. Low molecular weight 2,4-dinitrobenzenes are concentrated by isolated lymph node cells, but a representative 2,4-dinitrophenyl-protein conjugate (2,4-dinitrophenyl-bovine serum albumin) was not taken up to a detectable extent by these cells. It is inferred that there exist large quantitative differences in the extent to which dinitrophenyl-proteins are localized within cells following the administration to an intact animal of (a) those simple dinitrobenzenes which are both concentrated by lymph node cells and have the capacity to form protein conjugates in vivo, and (b) 2,4-dinitrophenyl-protein conjugates prepared in vitro. It is suggested that this difference could account for the fact that a varietyof 2,4-dinitrophenyl-proteins prepared in vitro are unable to induce contact skin sensitivity.
Article|
August 01 1959
A STUDY OF THE DISTRIBUTION OF 2,4-DINITROBENZENE SENSITIZERS BETWEEN ISOLATED LYMPH NODE CELLS AND EXTRACELLULAR MEDIUM IN RELATION TO INDUCTION OF CONTACT SKIN SENSITIVITY
Herman N. Eisen,
Herman N. Eisen
From the Division of Dermatology, Department of Internal Medicine, Washington University School of Medicine, and the Barnard Free Skin and Cancer Hospital, St. Louis
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Milton Kern,
Milton Kern
From the Division of Dermatology, Department of Internal Medicine, Washington University School of Medicine, and the Barnard Free Skin and Cancer Hospital, St. Louis
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William T. Newton,
William T. Newton
From the Division of Dermatology, Department of Internal Medicine, Washington University School of Medicine, and the Barnard Free Skin and Cancer Hospital, St. Louis
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Ernst Helmreich
Ernst Helmreich
From the Division of Dermatology, Department of Internal Medicine, Washington University School of Medicine, and the Barnard Free Skin and Cancer Hospital, St. Louis
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Herman N. Eisen
From the Division of Dermatology, Department of Internal Medicine, Washington University School of Medicine, and the Barnard Free Skin and Cancer Hospital, St. Louis
Milton Kern
From the Division of Dermatology, Department of Internal Medicine, Washington University School of Medicine, and the Barnard Free Skin and Cancer Hospital, St. Louis
William T. Newton
From the Division of Dermatology, Department of Internal Medicine, Washington University School of Medicine, and the Barnard Free Skin and Cancer Hospital, St. Louis
Ernst Helmreich
From the Division of Dermatology, Department of Internal Medicine, Washington University School of Medicine, and the Barnard Free Skin and Cancer Hospital, St. Louis
Received:
March 31 1959
Online Issn: 1540-9538
Print Issn: 0022-1007
Copyright, 1959, by The Rockefeller Institute
1959
J Exp Med (1959) 110 (2): 187–206.
Article history
Received:
March 31 1959
Citation
Herman N. Eisen, Milton Kern, William T. Newton, Ernst Helmreich; A STUDY OF THE DISTRIBUTION OF 2,4-DINITROBENZENE SENSITIZERS BETWEEN ISOLATED LYMPH NODE CELLS AND EXTRACELLULAR MEDIUM IN RELATION TO INDUCTION OF CONTACT SKIN SENSITIVITY . J Exp Med 1 August 1959; 110 (2): 187–206. doi: https://doi.org/10.1084/jem.110.2.187
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