The bactericidal substance present in mitochondrial extracts from rat leucocytes was found to be non-dialyzable, fractionated at 30 per cent (NH4SO4 saturation and was able to withstand temperatures up to 100°C.

The tentative identification of a lipoprotein as the responsible bactericidal substance is based on various observations; an ultraviolet absorption at a peak associated with alpha, beta unsaturated fatty acids, 205 to 208 mµ, inactivation of the bactericidal substance by ether treatment at –70°C. but not at 0°C. and inhibition of the bactericidal activity with lipase treatment as well as with trypsin.

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