The class II major histocompatibility complex genes all contain in their proximal promoters three cis-elements called S, X, and Y that are conserved in both sequence and position, and a fourth element, J, conserved in sequence but not in position. J, X, and Y and, to some extent, S, have been shown to be functionally important in regulation of expression of these genes. In the present study, a protein factor that binds cooperatively to the S plus J elements of the promoter of the class II major histocompatibility complex gene DPA has been detected. Moreover, functional cooperativity between S and J in activation of the enhancerless -40 interferon-beta (-40 IFN-beta) promoter has been demonstrated. Finally, the latter assay appears to subdivide complementation group A of class II negative human B cell lines that includes both mutants generated in vitro and cells from patients with the bare lymphocyte syndrome (type II). In three of these cell lines, the enhancerless -40 IFN-beta promoter containing the S plus J elements was functionally active, while in the others it was inactive.
Skip Nav Destination
Close
Article navigation
Article|
July 01 1995
Cooperativity between the J and S elements of class II major histocompatibility complex genes as enhancers in normal and class II-negative patient and mutant B cell lines.
M Sugawara
,
M Sugawara
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Search for other works by this author on:
T Scholl
,
T Scholl
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Search for other works by this author on:
S K Mahanta
,
S K Mahanta
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Search for other works by this author on:
P D Ponath
,
P D Ponath
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Search for other works by this author on:
J L Strominger
J L Strominger
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Search for other works by this author on:
M Sugawara
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
T Scholl
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
S K Mahanta
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
P D Ponath
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
J L Strominger
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1995) 182 (1): 175–184.
Citation
M Sugawara, T Scholl, S K Mahanta, P D Ponath, J L Strominger; Cooperativity between the J and S elements of class II major histocompatibility complex genes as enhancers in normal and class II-negative patient and mutant B cell lines.. J Exp Med 1 July 1995; 182 (1): 175–184. doi: https://doi.org/10.1084/jem.182.1.175
Download citation file:
Close
Advertisement