Nonirradiated B-lymphocyte-deficient CBA/N mice given T6T6 chromosome-marked normal CBA/CaHN spleen cells became lymphoid chimeras exhibiting donor-type mitoses. Normal CBA/CaHN recipients did not exhibit significant numbers of donor-type mitoses. The lymphoid cell chimerism in the CBA/N host appeared in spleen, lymph nodes, and Peyer's patches, but not in marrow or thymus. Stimulation of CBA/N-recipient spleen cells in vitro suggested that the chimerism involved donor T6T6 cells which were responsive to the B-lymphocyte mitogen, lipopolysaccharide, but not to the T-lymphocyte mitogen, phytohemagglutinin. These data indicate that stable, long-term chimerism of a specific class of lymphocytes is possible in nonirradiated, B-lymphocyte-deficient CBA/N mice.
Induction of partial chimerism in nonirradiated B-lymphocyte-deficient CBA/N mice.
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D Volf, L L Sensenbrenner, S J Sharkis, G J Elfenbein, I Scher; Induction of partial chimerism in nonirradiated B-lymphocyte-deficient CBA/N mice.. J Exp Med 1 March 1978; 147 (3): 940–945. doi: https://doi.org/10.1084/jem.147.3.940
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