The half disappearance time for detectable plasma membrane-associated and cytoplasmic immunoglobulin after treatment of continuously growing diploid lymphocytes with inhibitors of protein and RNA synthesis was studied. Also, the amount of plasma membrane-associated and cytoplasmic immunoglobulin of synchronized cells in the G1 phase of the cell cycle has been studied. Plasma membrane-associated immunoglobulin has a half disappearance time of 45 min after inhibition of protein synthesis. By contrast, after treatment of cells with actinomycin D for 24 hr, plasma membrane-associated immunoglobulin remains relatively unchanged whereas cytoplasmic immunoglobulin decreased by almost 90%. In the G1 phase of the cell cycle, plasma membrane-associated immunoglobulin and cytoplasmic immunoglobulin were 70 and 10%, respectively, of that in logarithmically growing cells, and the half disappearance of M-Ig after treatment of cells with puromycin was again 45 min. In toto, these results suggest that perhaps secreted and plasma membrane-associated immunoglobulin may be separately controlled by the cells.
SYNTHESIS OF PLASMA MEMBRANE-ASSOCIATED AND SECRETORY IMMUNOGLOBULIN IN DIPLOID LYMPHOCYTES
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Richard A. Lerner, Patricia J. McConahey, Inga Jansen, Frank J. Dixon; SYNTHESIS OF PLASMA MEMBRANE-ASSOCIATED AND SECRETORY IMMUNOGLOBULIN IN DIPLOID LYMPHOCYTES . J Exp Med 1 January 1972; 135 (1): 136–149. doi: https://doi.org/10.1084/jem.135.1.136
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