Type 1 innate lymphoid cells (ILC1s) represent the predominant population of liver ILCs and function as important effectors and regulators of immune responses, but the cellular heterogeneity of ILC1s is not fully understood. Here, single-cell RNA sequencing and flow cytometric analysis demonstrated that liver ILC1s could be dissected into Ly49E+ and Ly49E− populations with unique transcriptional and phenotypic features. Genetic fate-mapping analysis revealed that liver Ly49E+ ILC1s with strong cytotoxicity originated from embryonic non–bone marrow hematopoietic progenitor cells (HPCs), persisted locally during postnatal life, and mediated protective immunity against cytomegalovirus infection in newborn mice. However, Ly49E− ILC1s developed from BM and extramedullary HPCs after birth, gradually replaced Ly49E+ ILC1s in the livers with age, and contained the memory subset in recall response to hapten challenge. Thus, our study shows that Ly49E dissects liver ILC1s into two unique subpopulations, with distinct origins and a bias toward neonatal innate or adult immune memory responses.
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2 May 2022
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March 29 2022
Ly49E separates liver ILC1s into embryo-derived and postnatal subsets with different functions
Yawen Chen
,
(Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing - original draft)
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
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Xianwei Wang
,
(Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Validation, Visualization, Writing - original draft)
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
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Xiaolei Hao
,
Xiaolei Hao
(Software, Visualization)
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
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Bin Li
,
Bin Li
(Data curation, Formal analysis, Methodology, Software)
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
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Wanyin Tao
,
Wanyin Tao
(Methodology, Resources)
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
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Shu Zhu
,
Shu Zhu
(Methodology, Resources)
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
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Kun Qu
,
Kun Qu
(Data curation, Formal analysis, Funding acquisition, Software, Supervision)
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
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Haiming Wei
,
Haiming Wei
(Conceptualization)
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
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Rui Sun
,
Rui Sun
(Conceptualization, Data curation)
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
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Hui Peng
,
(Conceptualization, Funding acquisition, Project administration, Supervision, Validation, Visualization, Writing - original draft)
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Hui Peng: huipeng@ustc.edu.cn
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Zhigang Tian
(Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Visualization, Writing - original draft, Writing - review & editing)
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
2
Research Unit of NK Cell Study, Chinese Academy of Medical Sciences, Hefei, China
Correspondence to Zhigang Tian: tzg@ustc.edu.cn
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Yawen Chen
Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing - original draft
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Xianwei Wang
Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Validation, Visualization, Writing - original draft
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Xiaolei Hao
Software, Visualization
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Bin Li
Data curation, Formal analysis, Methodology, Software
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Wanyin Tao
Methodology, Resources
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Shu Zhu
Methodology, Resources
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Kun Qu
Data curation, Formal analysis, Funding acquisition, Software, Supervision
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Haiming Wei
Conceptualization
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Rui Sun
Conceptualization, Data curation
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Hui Peng
Conceptualization, Funding acquisition, Project administration, Supervision, Validation, Visualization, Writing - original draft
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Zhigang Tian
Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Visualization, Writing - original draft, Writing - review & editing
1
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Biomedical Sciences and Health Laboratory of Anhui Province, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
2
Research Unit of NK Cell Study, Chinese Academy of Medical Sciences, Hefei, China
Correspondence to Zhigang Tian: tzg@ustc.edu.cn
Hui Peng: huipeng@ustc.edu.cn
*
Y. Chen and X. Wang contributed equally to this paper.
Disclosures: The authors declare no competing interests exist.
Received:
August 25 2021
Revision Received:
January 23 2022
Accepted:
February 18 2022
Online Issn: 1540-9538
Print Issn: 0022-1007
Funding
Funder(s):
Natural Science Foundation of China
- Award Id(s): 81788101,92042305,81922029,31900637,81821001
Funder(s):
National Key R&D Program of China
- Award Id(s): 2020YFA0804100,2019YFA0508502/3
Funder(s):
CAMS Innovation Fund for Medical Sciences
- Award Id(s): 2019-I2M-5-073
© 2022 Chen et al.
2022
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
J Exp Med (2022) 219 (5): e20211805.
Article history
Received:
August 25 2021
Revision Received:
January 23 2022
Accepted:
February 18 2022
Citation
Yawen Chen, Xianwei Wang, Xiaolei Hao, Bin Li, Wanyin Tao, Shu Zhu, Kun Qu, Haiming Wei, Rui Sun, Hui Peng, Zhigang Tian; Ly49E separates liver ILC1s into embryo-derived and postnatal subsets with different functions. J Exp Med 2 May 2022; 219 (5): e20211805. doi: https://doi.org/10.1084/jem.20211805
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