Development and function of murine B220⁺CD11c⁺NK1.1⁺ cells identify them as a subset of NK cells

Lymphoid organs contain a B220⁺CD11c⁺NK1.1⁺ cell population that was recently characterized as a novel dendritic cell (DC) subset that functionally overlaps with natural killer (NK) cells and plasmacytoid DCs (PDCs). Using Siglec-H and NK1.1 markers, we unambiguously dissected B220⁺CD11c⁺ cells and found that PDCs are the only professional interferon (IFN)-α–producing cells within this heterogeneous population. In contrast, B220⁺CD11c⁺NK1.1⁺ cells are a discrete NK cell subset capable of producing higher levels of IFN-γ than conventional NK cells. Unlike DCs, only a minute fraction of B220⁺CD11c⁺NK1.1⁺ cells in the spleen expressed major histocompatibility complex class II ex vivo or after stimulation with CpG. Consistent with being a NK cell subset, B220⁺CD11c⁺NK1.1⁺ cells depended primarily on interleukin 15 and common cytokine receptor γ chain signaling for their development. In terms of function, expression of distinctive cell surface receptors, and location in lymphoid organs, NK1.1⁺B220⁺CD11c⁺ appear to be the murine equivalent of human CD56^bright NK cells.