Memory T cells can be divided into central–memory (TCM) and effector–memory (TEM) cells, which differ in their functional properties. Although both subpopulations can persist long term, it is not known whether they are maintained by similar mechanisms. We used in vivo labeling with deuterated glucose to measure the turnover of CD4+ T cells in healthy humans. The CD45R0+CCR7− TEM subpopulation was shown to have a rapid proliferation rate of 4.7% per day compared with 1.5% per day for CD45R0+CCR7+ TCM cells; these values are equivalent to average intermitotic (doubling) times of 15 and 48 d, respectively. In contrast, the CD45RA+CCR7+ naive CD4+ T cell population was found to be much longer lived, being labeled at a rate of only 0.2% per day (corresponding to an intermitotic time of approximately 1 yr). These data indicate that human CD4+ TEM cells constitute a short-lived cell population that requires continuous replenishment in vivo.
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19 July 2004
Brief Definitive Report|
July 12 2004
Rapid Turnover of Effector–Memory CD4+ T Cells in Healthy Humans
Derek C. Macallan,
Derek C. Macallan
1Department of Infectious Diseases, St. George's Hospital Medical School, London SW17 0RE, UK
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Diana Wallace,
Diana Wallace
2Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, UK
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Yan Zhang,
Yan Zhang
1Department of Infectious Diseases, St. George's Hospital Medical School, London SW17 0RE, UK
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Catherine de Lara,
Catherine de Lara
2Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, UK
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Andrew T. Worth,
Andrew T. Worth
2Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, UK
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Hala Ghattas,
Hala Ghattas
1Department of Infectious Diseases, St. George's Hospital Medical School, London SW17 0RE, UK
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George E. Griffin,
George E. Griffin
1Department of Infectious Diseases, St. George's Hospital Medical School, London SW17 0RE, UK
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Peter C.L. Beverley,
Peter C.L. Beverley
2Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, UK
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David F. Tough
David F. Tough
2Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, UK
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Derek C. Macallan
1Department of Infectious Diseases, St. George's Hospital Medical School, London SW17 0RE, UK
Diana Wallace
2Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, UK
Yan Zhang
1Department of Infectious Diseases, St. George's Hospital Medical School, London SW17 0RE, UK
Catherine de Lara
2Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, UK
Andrew T. Worth
2Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, UK
Hala Ghattas
1Department of Infectious Diseases, St. George's Hospital Medical School, London SW17 0RE, UK
George E. Griffin
1Department of Infectious Diseases, St. George's Hospital Medical School, London SW17 0RE, UK
Peter C.L. Beverley
2Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, UK
David F. Tough
2Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, UK
Address correspondence to David F. Tough, Edward Jenner Institute for Vaccine Research, High St., Compton, Newbury, Berkshire RG20 7NN, UK. Phone: 44-1635-577915; Fax: 44-1635-577901; email: [email protected]
Received:
February 20 2004
Accepted:
June 09 2004
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2004
J Exp Med (2004) 200 (2): 255–260.
Article history
Received:
February 20 2004
Accepted:
June 09 2004
Citation
Derek C. Macallan, Diana Wallace, Yan Zhang, Catherine de Lara, Andrew T. Worth, Hala Ghattas, George E. Griffin, Peter C.L. Beverley, David F. Tough; Rapid Turnover of Effector–Memory CD4+ T Cells in Healthy Humans . J Exp Med 19 July 2004; 200 (2): 255–260. doi: https://doi.org/10.1084/jem.20040341
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