Differentiation of naive CD4+ T cells into helper T (Th) cells is controlled by a combination of several transcriptional factors. In this study, we examined the functional role of the Runx1 transcription factor in Th cell differentiation. Naive T cells from transgenic mice expressing a dominant interfering form of Runx1 exhibited enhanced interleukin 4 production and efficient Th2 differentiation. In contrast, transduction of Runx1 into wild-type T cells caused a complete attenuation of Th2 differentiation and was accompanied by the cessation of GATA3 expression. Furthermore, endogenous expression of Runx1 in naive T cells declined after T cell receptor stimulation, at the same time that expression of GATA3 increased. We conclude that Runx1 plays a novel role as a negative regulator of GATA3 expression, thereby inhibiting the Th2 cell differentiation.
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7 July 2003
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June 30 2003
The Runx1 Transcription Factor Inhibits the Differentiation of Naive CD4 + T Cells into the Th2 Lineage by Repressing GATA3 Expression
Okiru Komine,
Okiru Komine
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
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Keitaro Hayashi,
Keitaro Hayashi
2Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan
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Waka Natsume,
Waka Natsume
2Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan
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Toshio Watanabe,
Toshio Watanabe
2Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan
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Youichi Seki,
Youichi Seki
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
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Noriyasu Seki,
Noriyasu Seki
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
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Ryoji Yagi,
Ryoji Yagi
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
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Wataru Sukzuki,
Wataru Sukzuki
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
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Hidekazu Tamauchi,
Hidekazu Tamauchi
3Department of Microbiology plus Parasitology, School of Medicine, Kitasato University, Sagamihara 228-8555, Japan
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Katsuto Hozumi,
Katsuto Hozumi
4Department of Immunology, Tokai University School of Medicine, Boseidai, Isehara 259-1193, Japan
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Sonoko Habu,
Sonoko Habu
4Department of Immunology, Tokai University School of Medicine, Boseidai, Isehara 259-1193, Japan
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Masato Kubo,
Masato Kubo
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
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Masanobu Satake
Masanobu Satake
2Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan
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Okiru Komine
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
Keitaro Hayashi
2Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan
Waka Natsume
2Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan
Toshio Watanabe
2Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan
Youichi Seki
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
Noriyasu Seki
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
Ryoji Yagi
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
Wataru Sukzuki
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
Hidekazu Tamauchi
3Department of Microbiology plus Parasitology, School of Medicine, Kitasato University, Sagamihara 228-8555, Japan
Katsuto Hozumi
4Department of Immunology, Tokai University School of Medicine, Boseidai, Isehara 259-1193, Japan
Sonoko Habu
4Department of Immunology, Tokai University School of Medicine, Boseidai, Isehara 259-1193, Japan
Masato Kubo
1Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan
Masanobu Satake
2Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan
Address correspondence to Masanobu Satake, Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. Phone: 81-22-717-8481; Fax: 81-22-717-8482; E-mail: [email protected]; or Masato Kubo, Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda 278-0022, Japan. Phone: 81-4-7121-4060; Fax: 81-4-7121-4069; E-mail: [email protected]
O. Komine and K. Hayashi contributed equally to this work.
*
Abbreviation used in this paper: GFP, green fluorescent protein.
Received:
July 16 2002
Revision Received:
April 02 2003
Accepted:
April 15 2003
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2003
J Exp Med (2003) 198 (1): 51–61.
Article history
Received:
July 16 2002
Revision Received:
April 02 2003
Accepted:
April 15 2003
Citation
Okiru Komine, Keitaro Hayashi, Waka Natsume, Toshio Watanabe, Youichi Seki, Noriyasu Seki, Ryoji Yagi, Wataru Sukzuki, Hidekazu Tamauchi, Katsuto Hozumi, Sonoko Habu, Masato Kubo, Masanobu Satake; The Runx1 Transcription Factor Inhibits the Differentiation of Naive CD4+ T Cells into the Th2 Lineage by Repressing GATA3 Expression . J Exp Med 7 July 2003; 198 (1): 51–61. doi: https://doi.org/10.1084/jem.20021200
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