Common lymphoid progenitors (CLP) are generated in adult bone marrow (BM), but the intermediate steps leading to T cell commitment are unknown, and so is the site at which this commitment occurs. Here, we show that colonies arising in the spleen 12 days after BM injection harbor T cell precursors that are undetectable in BM. These precursors did not generate myeloid cells in vivo but repopulated the thymus and the peripheral T cell compartment much faster than did CLP. Two lineage negative (Lin−) subpopulations were distinguished, namely CD44+ Thy1− cells still capable of natural killer generation and transient low-level B cell generation, and T cell–restricted CD44− Thy1+ cells. At a molecular level, frequency of CD3ε and preTα mRNA was very different in each subset. Furthermore, only the CD44− Thy1+ subset have initiated rearrangements in the T cell receptor β locus. Thus, this study identifies extramedullary T cell progenitors and will allow easy approach to T cell commitment studies.
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1 April 2002
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April 01 2002
Major T Cell Progenitor Activity in Bone Marrow–derived Spleen Colonies
Christophe Lancrin,
Christophe Lancrin
1INSERM U345, Institut Necker
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Elke Schneider,
Elke Schneider
2CNRS UMR 8603, Hôpital Necker
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Florence Lambolez,
Florence Lambolez
1INSERM U345, Institut Necker
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Marie-Laure Arcangeli,
Marie-Laure Arcangeli
1INSERM U345, Institut Necker
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Corinne Garcia-Cordier,
Corinne Garcia-Cordier
3IFR 94 (Institut Fédératif de Recherche Necker-Enfants-Malades), 75730 Paris Cedex 15, France
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Benedita Rocha,
Benedita Rocha
1INSERM U345, Institut Necker
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Sophie Ezine
Sophie Ezine
1INSERM U345, Institut Necker
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Christophe Lancrin
1INSERM U345, Institut Necker
Elke Schneider
2CNRS UMR 8603, Hôpital Necker
Florence Lambolez
1INSERM U345, Institut Necker
Marie-Laure Arcangeli
1INSERM U345, Institut Necker
Corinne Garcia-Cordier
3IFR 94 (Institut Fédératif de Recherche Necker-Enfants-Malades), 75730 Paris Cedex 15, France
Benedita Rocha
1INSERM U345, Institut Necker
Sophie Ezine
1INSERM U345, Institut Necker
Address correspondence to S. Ezine, INSERM U345, Institut Necker, 156 rue de Vaugirard, 75730 Paris, Cedex 15, France. Phone: 00-33-14-061-5365; Fax: 00-33-14-061-5580; E-mail: [email protected]
*
Abbreviations used in this paper: BM, bone marrow; CLP, common lymphoid progenitor; HSC, hematopoietic stem cell; MPP, multipotent progenitor; Rag, recombination activating gene; SC12, day 12 spleen colonies; Tx, thymectomized.
Received:
August 27 2001
Revision Received:
February 06 2002
Accepted:
February 22 2002
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2002
J Exp Med (2002) 195 (7): 919–929.
Article history
Received:
August 27 2001
Revision Received:
February 06 2002
Accepted:
February 22 2002
Citation
Christophe Lancrin, Elke Schneider, Florence Lambolez, Marie-Laure Arcangeli, Corinne Garcia-Cordier, Benedita Rocha, Sophie Ezine; Major T Cell Progenitor Activity in Bone Marrow–derived Spleen Colonies . J Exp Med 1 April 2002; 195 (7): 919–929. doi: https://doi.org/10.1084/jem.20011475
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