We recently described a novel way to isolate populations of antigen-reactive CD4+ T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS® sorting by CD4high expression. Phenotypic, FACS®, functional, antibody inhibition, and major histocompatibility complex–peptide tetramer analyses, as well as T cell receptor Vβ sequence analyses, of the antigen-specific CD4high T cell populations demonstrated that a diverse sperm whale myoglobin 110–121–reactive CD4+ T cell repertoire was activated at the beginning (day 3 after immunization) of the immune response. Within 6 d of immunization, lower affinity clones were lost from the responding population, leaving an expanded population of oligoclonal, intermediate affinity (and residual high affinity) T cells. This T cell subset persisted for at least 4 wk after immunization and dominated the secondary immune response. These data provide evidence that CD4+ T cell repertoire selection occurs early in the immune response in vivo and suggest that persistence and expansion of a population of oligoclonal, intermediate affinity T cells is involved in CD4+ T cell memory.
Skip Nav Destination
Article navigation
18 December 2000
Article|
December 11 2000
T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo
Marcella Fassò,
Marcella Fassò
aDepartment of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, California 94305
Search for other works by this author on:
Niroshana Anandasabapathy,
Niroshana Anandasabapathy
aDepartment of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, California 94305
Search for other works by this author on:
Frances Crawford,
Frances Crawford
cDepartment of Immunology, Howard Hughes Medical Institute, National Jewish Medical and Research Center and University of Colorado School of Medicine, Denver, Colorado 80206
Search for other works by this author on:
John Kappler,
John Kappler
cDepartment of Immunology, Howard Hughes Medical Institute, National Jewish Medical and Research Center and University of Colorado School of Medicine, Denver, Colorado 80206
Search for other works by this author on:
C. Garrison Fathman,
C. Garrison Fathman
aDepartment of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, California 94305
Search for other works by this author on:
William M. Ridgway
William M. Ridgway
bDepartment of Medicine, Division of Rheumatology and Immunology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261
Search for other works by this author on:
Marcella Fassò
aDepartment of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, California 94305
Niroshana Anandasabapathy
aDepartment of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, California 94305
Frances Crawford
cDepartment of Immunology, Howard Hughes Medical Institute, National Jewish Medical and Research Center and University of Colorado School of Medicine, Denver, Colorado 80206
John Kappler
cDepartment of Immunology, Howard Hughes Medical Institute, National Jewish Medical and Research Center and University of Colorado School of Medicine, Denver, Colorado 80206
C. Garrison Fathman
aDepartment of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, California 94305
William M. Ridgway
bDepartment of Medicine, Division of Rheumatology and Immunology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261
Abbreviations used in this paper: Bio, biotin; LDA, limiting dilution analysis; PCC, pigeon cytochrome c; SWM, sperm whale myoglobin.
Received:
June 09 2000
Revision Requested:
September 27 2000
Accepted:
October 07 2000
Online ISSN: 1540-9538
Print ISSN: 0022-1007
© 2000 The Rockefeller University Press
2000
The Rockefeller University Press
J Exp Med (2000) 192 (12): 1719–1730.
Article history
Received:
June 09 2000
Revision Requested:
September 27 2000
Accepted:
October 07 2000
Citation
Marcella Fassò, Niroshana Anandasabapathy, Frances Crawford, John Kappler, C. Garrison Fathman, William M. Ridgway; T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo. J Exp Med 18 December 2000; 192 (12): 1719–1730. doi: https://doi.org/10.1084/jem.192.12.1719
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement