The B cell antigen receptor (BCR) is a large complex that consists of a disulfide-linked tetramer of two transmembrane heavy (μ) chains and two light (λ or κ) chains in association with a heterodimer of Igα and Igβ. Kaposi's sarcoma–associated herpesvirus (KSHV) encodes a transforming protein called K1, which has structural and functional similarity to Igα and Igβ. We demonstrate that K1 downregulates the expression of BCR complexes on the surface. The NH2-terminal region of K1 specifically interacts with the μ chains of BCR complexes, and this interaction retains BCR complexes in the endoplasmic reticulum, preventing their intracellular transport to the cell surface. Thus, KSHV K1 resembles Igα and Igβ in its ability to induce signaling and to interact with μ chains of the BCR. However, unlike Igα and Igβ, which interact with μ chains to direct BCR complexes to the cell surface, K1 interacts with μ chains to block the intracellular transport of BCR complexes to the cell surface. These results demonstrate a unique feature of the K1 transforming protein, which may confer virus-infected cells with a long-term survival advantage.
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3 July 2000
Article|
June 26 2000
Inhibition of Intracellular Transport of B Cell Antigen Receptor Complexes by Kaposi's Sarcoma–Associated Herpesvirus K1
Bok-Soo Lee,
Bok-Soo Lee
aDepartment of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
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Xavier Alvarez,
Xavier Alvarez
bDepartment of Pathology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
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Satoshi Ishido,
Satoshi Ishido
aDepartment of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
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Andrew A. Lackner,
Andrew A. Lackner
bDepartment of Pathology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
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Jae U. Jung
Jae U. Jung
aDepartment of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
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Bok-Soo Lee
aDepartment of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
Xavier Alvarez
bDepartment of Pathology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
Satoshi Ishido
aDepartment of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
Andrew A. Lackner
bDepartment of Pathology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
Jae U. Jung
aDepartment of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
Abbreviations used in this paper: BCR, B cell antigen receptor; ER, endoplasmic reticulum; GST, glutathione S-transferase; ITAM, immunoreceptor tyrosine-based activation motif; KSHV, Kaposi's sarcoma–associated herpesvirus; LMP2A, latent membrane protein 2A; PEL, primary effusion lymphoma.
Received:
February 24 2000
Revision Requested:
April 12 2000
Accepted:
May 01 2000
Online ISSN: 1540-9538
Print ISSN: 0022-1007
© 2000 The Rockefeller University Press
2000
The Rockefeller University Press
J Exp Med (2000) 192 (1): 11–22.
Article history
Received:
February 24 2000
Revision Requested:
April 12 2000
Accepted:
May 01 2000
Citation
Bok-Soo Lee, Xavier Alvarez, Satoshi Ishido, Andrew A. Lackner, Jae U. Jung; Inhibition of Intracellular Transport of B Cell Antigen Receptor Complexes by Kaposi's Sarcoma–Associated Herpesvirus K1. J Exp Med 3 July 2000; 192 (1): 11–22. doi: https://doi.org/10.1084/jem.192.1.11
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