The M88.7 T cell clone recognizes an antigen presented by HLA B*1302 on the melanoma cell line M88. A cDNA encoding this antigen (NA88-A) was isolated using a library transfection approach. Analysis of the genomic gene's sequence identified it is a processed pseudogene, derived from a retrotranscript of mRNA coding for homeoprotein HPX42B. The NA88-A gene exhibits several premature stop codons, deletions, and insertions relative to the HPX42B gene. In NA88-A RNA, a short open reading frame codes for the peptide MTQGQHFLQKV from which antigenic peptides are derived; a stop codon follows the peptide's COOH-terminal Val codon. Part of the HPX42B mRNA's 3′ untranslated region codes for a peptide of similar sequence (MTQGQHFSQKV). If produced, this peptide can be recognized by M88.7 T cells. However, in HPX42B mRNA, the peptide's COOH-terminal Val codon is followed by a Trp codon. As a result, expression of HPX42B mRNA does not lead to antigen production. A model is proposed for events that participated in creation of a gene coding for a melanoma antigen from a pseudogene.
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1 May 2000
Brief Definitive Report|
May 01 2000
A Processed Pseudogene Codes for a New Antigen Recognized by a Cd8+ T Cell Clone on Melanoma
Agnès Moreau-Aubry,
Agnès Moreau-Aubry
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
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Soizic Le Guiner,
Soizic Le Guiner
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
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Nathalie Labarrière,
Nathalie Labarrière
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
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Marie-Claude Gesnel,
Marie-Claude Gesnel
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
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Francine Jotereau,
Francine Jotereau
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
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Richard Breathnach
Richard Breathnach
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
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Agnès Moreau-Aubry
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
Soizic Le Guiner
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
Nathalie Labarrière
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
Marie-Claude Gesnel
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
Francine Jotereau
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
Richard Breathnach
aInstitut National de la Santé et de la Recherche Médicale (INSERM) U463, 44093 Nantes cedex 01, France
A. Moreau-Aubry and S. Le Guiner contributed equally to this work.
Received:
February 22 2000
Accepted:
March 13 2000
Online ISSN: 1540-9538
Print ISSN: 0022-1007
© 2000 The Rockefeller University Press
2000
The Rockefeller University Press
J Exp Med (2000) 191 (9): 1617–1624.
Article history
Received:
February 22 2000
Accepted:
March 13 2000
Citation
Agnès Moreau-Aubry, Soizic Le Guiner, Nathalie Labarrière, Marie-Claude Gesnel, Francine Jotereau, Richard Breathnach; A Processed Pseudogene Codes for a New Antigen Recognized by a Cd8+ T Cell Clone on Melanoma. J Exp Med 1 May 2000; 191 (9): 1617–1624. doi: https://doi.org/10.1084/jem.191.9.1617
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