The distribution of immunoglobulin (Ig) isotypes within specific B cell clones in vivo after immunization is not well defined. Using an IgVH/CDR3- and isotype-specific reverse transcription polymerase chain reaction method, we have carried out a survey of the diversification of the isotype in a splenic response to phenyl-oxazolone (phOx) on a chicken serum albumin carrier. The phOx-specific VH (VHOx-1 with specific CDR3 motif) is associated with all of the heavy chains (μ, δ, α, γ, and ε) after simple immunization with antigen in alum. The kinetics of expression of each isotype are distinct and reproducible. Focusing mainly on the expression of secretory Ig transcripts, IgM, IgG1, and IgE are found after priming, whereas IgD and IgA appear after boosting. Secretory IgD transcripts are found reproducibly at moderate levels and may, therefore, contribute significantly to the secreted Ig response in mice. Most crucially, we find enhanced levels of secretory IgM/VHOx-1 transcripts (with ‘phOx-specific’ CDR3) after boosting, strongly indicating the existence of IgM memory cells that give rise to an enhanced specific IgM secretion in the secondary response.
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19 June 2000
Article|
June 19 2000
Analysis of Immunoglobulin (Ig) Isotype Diversity and Igm/D Memory in the Response to Phenyl-Oxazolone
Harry White,
Harry White
aDepartment of Molecular Immunology, Institute of Child Health, London WC1N 1EH, United Kingdom
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David Gray
David Gray
bInstitute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom
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Harry White
aDepartment of Molecular Immunology, Institute of Child Health, London WC1N 1EH, United Kingdom
David Gray
bInstitute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom
Abbreviations used in this paper: CSA, chicken serum albumin; mem, membrane; phOx, phenyl-oxazolone; RT, reverse transcription; sec, secretory.
Received:
December 20 1999
Revision Requested:
April 05 2000
Accepted:
April 17 2000
Online ISSN: 1540-9538
Print ISSN: 0022-1007
© 2000 The Rockefeller University Press
2000
The Rockefeller University Press
J Exp Med (2000) 191 (12): 2209–2220.
Article history
Received:
December 20 1999
Revision Requested:
April 05 2000
Accepted:
April 17 2000
Citation
Harry White, David Gray; Analysis of Immunoglobulin (Ig) Isotype Diversity and Igm/D Memory in the Response to Phenyl-Oxazolone. J Exp Med 19 June 2000; 191 (12): 2209–2220. doi: https://doi.org/10.1084/jem.191.12.2209
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