A panel of cDNAs encoding allergenic proteins was isolated from an Aspergillus fumigatus cDNA library displayed on the surface of filamentous phage. Solid phase–immobilized serum immunoglobulin E (IgE) from A. fumigatus–allergic individuals was used to enrich phage displaying IgE-binding molecules. One of the cDNAs encoded a 11.1-kD protein that was identified as acidic ribosomal phosphoprotein type 2 (P2 protein). The allergen, formally termed rAsp f 8, shares >62% sequence identity and >84% sequence homology to corresponding eukaryotic P2 proteins, including human P2 protein. The sequences encoding human and fungal P2 protein were subcloned, expressed in Escherichia coli as His6-tagged fusion proteins, and purified by Ni2+–chelate affinity chromatography. Both recombinant P2 proteins were recognized by IgE antibodies from allergic individuals sensitized to the A. fumigatus P2 protein and elicited strong type 1–specific skin reactions in these individuals. Moreover, human and fungal P2 proteins induced proliferative responses in peripheral blood mononuclear cells of A. fumigatus– allergic subjects sensitized to the fungal P2 protein. These data provide strong evidence for in vitro and in vivo humoral and cell-mediated autoreactivity to human P2 protein in patients suffering from chronic A. fumigatus allergy.
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3 May 1999
Brief Definitive Report|
May 03 1999
Humoral and Cell-mediated Autoimmune Reactions to Human Acidic Ribosomal P2 Protein in Individuals Sensitized to Aspergillus fumigatus P2 Protein
Christina Mayer,
Christina Mayer
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
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Ulrich Appenzeller,
Ulrich Appenzeller
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
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Heike Seelbach,
Heike Seelbach
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
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Gernot Achatz,
Gernot Achatz
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
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Hannes Oberkofler,
Hannes Oberkofler
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
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Michael Breitenbach,
Michael Breitenbach
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
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Kurt Blaser,
Kurt Blaser
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
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Reto Crameri
Reto Crameri
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
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Christina Mayer
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
Ulrich Appenzeller
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
Heike Seelbach
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
Gernot Achatz
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
Hannes Oberkofler
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
Michael Breitenbach
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
Kurt Blaser
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
Reto Crameri
From the *Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; ‡Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the §Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria
Address correspondence to Reto Crameri, Swiss Institute of Allergy and Asthma Research (SIAF), Obere Strasse 22, CH-7270 Davos Platz, Switzerland. Phone: 41-81-410-08-48; Fax: 41-81-410-08-40; E-mail: [email protected]
Received:
February 02 1999
Revision Received:
March 05 1999
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1999
J Exp Med (1999) 189 (9): 1507–1512.
Article history
Received:
February 02 1999
Revision Received:
March 05 1999
Citation
Christina Mayer, Ulrich Appenzeller, Heike Seelbach, Gernot Achatz, Hannes Oberkofler, Michael Breitenbach, Kurt Blaser, Reto Crameri; Humoral and Cell-mediated Autoimmune Reactions to Human Acidic Ribosomal P2 Protein in Individuals Sensitized to Aspergillus fumigatus P2 Protein . J Exp Med 3 May 1999; 189 (9): 1507–1512. doi: https://doi.org/10.1084/jem.189.9.1507
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