Secondary lymphoid organ chemokine (SLC) is expressed in high endothelial venules and in T cell zones of spleen and lymph nodes (LNs) and strongly attracts naive T cells. In mice homozygous for the paucity of lymph node T cell (plt) mutation, naive T cells fail to home to LNs or the lymphoid regions of spleen. Here we demonstrate that expression of SLC is undetectable in plt mice. In addition to the defect in T cell homing, we demonstrate that dendritic cells (DCs) fail to accumulate in spleen and LN T cell zones of plt mice. DC migration to LNs after contact sensitization is also substantially reduced. The physiologic significance of these abnormalities in plt mice is indicated by a markedly increased sensitivity to infection with murine hepatitis virus. The plt mutation maps to the SLC locus; however, the sequence of SLC introns and exons in plt mice is normal. These findings suggest that the abnormalities in plt mice are due to a genetic defect in the expression of SLC and that SLC mediates the entry of naive T cells and antigen-stimulated DCs into the T cell zones of secondary lymphoid organs.
Skip Nav Destination
Article navigation
1 February 1999
Article|
February 01 1999
Mice Lacking Expression of Secondary Lymphoid Organ Chemokine Have Defects in Lymphocyte Homing and Dendritic Cell Localization
Michael D. Gunn,
Michael D. Gunn
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Search for other works by this author on:
Shigeru Kyuwa,
Shigeru Kyuwa
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Search for other works by this author on:
Carmen Tam,
Carmen Tam
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Search for other works by this author on:
Terutaka Kakiuchi,
Terutaka Kakiuchi
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Search for other works by this author on:
Akio Matsuzawa,
Akio Matsuzawa
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Search for other works by this author on:
Lewis T. Williams,
Lewis T. Williams
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Search for other works by this author on:
Hideki Nakano
Hideki Nakano
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Search for other works by this author on:
Michael D. Gunn
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Shigeru Kyuwa
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Carmen Tam
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Terutaka Kakiuchi
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Akio Matsuzawa
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Lewis T. Williams
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Hideki Nakano
From the *Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143; the ‡Center for Experimental Medicine and §Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan; and the ‖Department of Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
Address correspondence to Michael Dee Gunn, Division of Cardiology, Duke University Medical Center, Box 3547, Durham, NC 27710. Phone: 919-681-5072; Fax: 919-684-8591; E-mail: [email protected]
Received:
September 17 1998
Revision Received:
November 24 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1999
J Exp Med (1999) 189 (3): 451–460.
Article history
Received:
September 17 1998
Revision Received:
November 24 1998
Citation
Michael D. Gunn, Shigeru Kyuwa, Carmen Tam, Terutaka Kakiuchi, Akio Matsuzawa, Lewis T. Williams, Hideki Nakano; Mice Lacking Expression of Secondary Lymphoid Organ Chemokine Have Defects in Lymphocyte Homing and Dendritic Cell Localization . J Exp Med 1 February 1999; 189 (3): 451–460. doi: https://doi.org/10.1084/jem.189.3.451
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
