PIR-A and PIR-B, paired immunoglobulin-like receptors encoded, respectively, by multiple Pira genes and a single Pirb gene in mice, are relatives of the human natural killer (NK) and Fc receptors. Monoclonal and polyclonal antibodies produced against a recombinant PIR protein identified cell surface glycoproteins of ∼85 and ∼120 kD on B cells, granulocytes, and macrophages. A disulfide-linked homodimer associated with the cell surface PIR molecules was identified as the Fc receptor common γ (FcRγc) chain. Whereas PIR-B fibroblast transfectants expressed cell surface molecules of ∼120 kD, PIR-A transfectants expressed the ∼85-kD molecules exclusively intracellularly; PIR-A and FcRγc cotransfectants expressed the PIR-A/ FcRγc complex on their cell surface. Correspondingly, PIR-B was normally expressed on the cell surface of splenocytes from FcRγc−/− mice whereas PIR-A was not. Cell surface levels of PIR molecules on myeloid and B lineage cells increased with cellular differentiation and activation. Dendritic cells, monocytes/macrophages, and mast cells expressed the PIR molecules in varying levels, but T cells and NK cells did not. These experiments define the coordinate cellular expression of PIR-B, an inhibitory receptor, and PIR-A, an activating receptor; demonstrate the requirement of FcRγc chain association for cell surface PIR-A expression; and suggest that the level of FcRγc chain expression could differentially affect the PIR-A/PIR-B equilibrium in different cell lineages.
Skip Nav Destination
Article navigation
18 January 1999
Article|
January 18 1999
Biochemical Nature and Cellular Distribution of the Paired Immunoglobulin-like Receptors, PIR-A and PIR-B
Hiromi Kubagawa,
Hiromi Kubagawa
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Search for other works by this author on:
Ching-Cheng Chen,
Ching-Cheng Chen
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Search for other works by this author on:
Le Hong Ho,
Le Hong Ho
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Search for other works by this author on:
Toshihide Shimada,
Toshihide Shimada
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Search for other works by this author on:
Lanier Gartland,
Lanier Gartland
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Search for other works by this author on:
Charles Mashburn,
Charles Mashburn
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Search for other works by this author on:
Takahiro Uehara,
Takahiro Uehara
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Search for other works by this author on:
Jeffrey V. Ravetch,
Jeffrey V. Ravetch
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Search for other works by this author on:
Max D. Cooper
Max D. Cooper
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Search for other works by this author on:
Hiromi Kubagawa
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Ching-Cheng Chen
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Le Hong Ho
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Toshihide Shimada
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Lanier Gartland
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Charles Mashburn
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Takahiro Uehara
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Jeffrey V. Ravetch
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Max D. Cooper
From the Division of Developmental and Clinical Immunology, *Department of Pathology, ‡Department of Microbiology, §Department of Pediatrics, and ‖Department of Medicine, University of Alabama at Birmingham, and the ¶Howard Hughes Medical Institute, Birmingham, Alabama 35294; and the **Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York 10021
Address correspondence to Hiromi Kubagawa, 378 WTI, Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294-3300; Phone: 205-934-3370; Fax: 205-975-7218; E-mail: [email protected]
H. Kubagawa and C.-C. Chen contributed equally to this work.
Received:
August 31 1998
Revision Received:
November 02 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1999
J Exp Med (1999) 189 (2): 309–318.
Article history
Received:
August 31 1998
Revision Received:
November 02 1998
Citation
Hiromi Kubagawa, Ching-Cheng Chen, Le Hong Ho, Toshihide Shimada, Lanier Gartland, Charles Mashburn, Takahiro Uehara, Jeffrey V. Ravetch, Max D. Cooper; Biochemical Nature and Cellular Distribution of the Paired Immunoglobulin-like Receptors, PIR-A and PIR-B . J Exp Med 18 January 1999; 189 (2): 309–318. doi: https://doi.org/10.1084/jem.189.2.309
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
