We have used intravital microscopy to study physiologically perfused microvessels in murine bone marrow (BM). BM sinusoids and venules, but not adjacent bone vessels, supported rolling interactions of hematopoietic progenitor cells. Rolling did not involve L-selectin, but was partially reduced in wild-type mice treated with antibodies to P- or E-selectin and in mice that were deficient in these two selectins. Selectin-independent rolling was mediated by α4 integrins, which interacted with endothelial vascular cell adhesion molecule (VCAM)-1. Parallel contribution of the endothelial selectins and VCAM-1 is not known to direct blood cell trafficking to other noninflamed tissues. This combination of constitutively expressed adhesion molecules may thus constitute a BM-specific recruitment pathway for progenitor cells analogous to the vascular addressins that direct selective lymphocyte homing to lymphoid organs.
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3 August 1998
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August 03 1998
Hematopoietic Progenitor Cell Rolling in Bone Marrow Microvessels: Parallel Contributions by Endothelial Selectins and Vascular Cell Adhesion Molecule 1
Irina B. Mazo,
Irina B. Mazo
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
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Jose-Carlos Gutierrez-Ramos,
Jose-Carlos Gutierrez-Ramos
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
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Paul S. Frenette,
Paul S. Frenette
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
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Richard O. Hynes,
Richard O. Hynes
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
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Denisa D. Wagner,
Denisa D. Wagner
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
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Ulrich H. von Andrian
Ulrich H. von Andrian
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
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Irina B. Mazo
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Jose-Carlos Gutierrez-Ramos
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Paul S. Frenette
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Richard O. Hynes
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Denisa D. Wagner
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Ulrich H. von Andrian
From *The Center for Blood Research, the ‡Department of Pathology, and the §Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; ‖Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139; and the ¶Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Address correspondence to Ulrich H. von Andrian, The Center for Blood Research, 200 Longwood Ave., Boston, MA 02115. Phone: 617-278-3130; Fax: 617-278-3190; E-mail: [email protected]
Received:
March 31 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1998
J Exp Med (1998) 188 (3): 465–474.
Article history
Received:
March 31 1998
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Citation
Irina B. Mazo, Jose-Carlos Gutierrez-Ramos, Paul S. Frenette, Richard O. Hynes, Denisa D. Wagner, Ulrich H. von Andrian; Hematopoietic Progenitor Cell Rolling in Bone Marrow Microvessels: Parallel Contributions by Endothelial Selectins and Vascular Cell Adhesion Molecule 1 . J Exp Med 3 August 1998; 188 (3): 465–474. doi: https://doi.org/10.1084/jem.188.3.465
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