Mouse germinal center (GC) B cells have been shown to undergo secondary V(D)J (V, variable; D, diversity; J, joining) recombination (receptor editing) mediated by the reexpressed products of recombination activating gene (RAG)-1 and RAG-2. We show here that interleukin (IL)-7 as well as IL-4 was effective in inducing functional RAG products in mouse IgD+ B cells activated via CD40 in vitro. Blocking of the IL-7 receptor (IL-7R) by injecting an anti– IL-7R monoclonal antibody resulted in a marked suppression of the reexpression of RAG-2 and subsequent V(D)J recombination in the draining lymph node of immunized mice, whereas RAG-2 expression was not impaired in immunized IL-4–deficient mice. Further, these peripheral B cells activated in vitro or in vivo were found to express IL-7R. These findings indicate a novel role for IL-7 and IL-7R in inducing receptor editing in GC B cells.
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20 July 1998
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July 20 1998
Expression of Recombination Activating Genes in Germinal Center B Cells: Involvement of Interleukin 7 (IL-7) and the IL-7 Receptor
Masaki Hikida,
Masaki Hikida
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
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Yasunori Nakayama,
Yasunori Nakayama
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
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Yumi Yamashita,
Yumi Yamashita
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
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Yoshio Kumazawa,
Yoshio Kumazawa
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
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Shin-Ichi Nishikawa,
Shin-Ichi Nishikawa
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
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Hitoshi Ohmori
Hitoshi Ohmori
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
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Masaki Hikida
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
Yasunori Nakayama
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
Yumi Yamashita
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
Yoshio Kumazawa
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
Shin-Ichi Nishikawa
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
Hitoshi Ohmori
From the *Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan; the ‡Department of Biosciences, School of Science, Kitasato University, Kanagawa 228, Japan; and the §Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
Address correspondence to Hitoshi Ohmori, Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan. Phone and Fax: 81-86-251-8197; E-mail: [email protected]
Received:
March 20 1998
Revision Received:
May 15 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1998
J Exp Med (1998) 188 (2): 365–372.
Article history
Received:
March 20 1998
Revision Received:
May 15 1998
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Masaki Hikida, Yasunori Nakayama, Yumi Yamashita, Yoshio Kumazawa, Shin-Ichi Nishikawa, Hitoshi Ohmori; Expression of Recombination Activating Genes in Germinal Center B Cells: Involvement of Interleukin 7 (IL-7) and the IL-7 Receptor . J Exp Med 20 July 1998; 188 (2): 365–372. doi: https://doi.org/10.1084/jem.188.2.365
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