We explore here if vaccination with DNA encoding an autoantigenic peptide can suppress autoimmune disease. For this purpose we used experimental autoimmune encephalomyelitis (EAE), which is an autoaggressive disease in the central nervous system and an animal model for multiple sclerosis. Lewis rats were vaccinated with DNA encoding an encephalitogenic T cell epitope, guinea pig myelin basic protein peptide 68–85 (MBP68–85), before induction of EAE with MBP68–85 in complete Freund's adjuvant. Compared to vaccination with a control DNA construct, the vaccination suppressed clinical and histopathological signs of EAE, and reduced the interferon γ production after challenge with MBP68–85. Targeting of the gene product to Fc of IgG was essential for this effect. There were no signs of a Th2 cytokine bias. Our data suggest that DNA vaccines encoding autoantigenic peptides may be useful tools in controlling autoimmune disease.
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4 May 1998
Brief Definitive Report|
May 04 1998
Vaccination with DNA Encoding an Immunodominant Myelin Basic Protein Peptide Targeted to Fc of Immunoglobulin G Suppresses Experimental Autoimmune Encephalomyelitis
Anna Lobell,
Anna Lobell
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
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Robert Weissert,
Robert Weissert
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
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Maria K. Storch,
Maria K. Storch
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
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Cecilia Svanholm,
Cecilia Svanholm
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
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Katrien L. de Graaf,
Katrien L. de Graaf
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
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Hans Lassmann,
Hans Lassmann
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
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Roland Andersson,
Roland Andersson
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
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Tomas Olsson,
Tomas Olsson
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
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Hans Wigzell
Hans Wigzell
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
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Anna Lobell
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
Robert Weissert
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
Maria K. Storch
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
Cecilia Svanholm
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
Katrien L. de Graaf
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
Hans Lassmann
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
Roland Andersson
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
Tomas Olsson
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
Hans Wigzell
From the *Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden; the ‡Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden; and the §Neurological Institute, University of Vienna, A-1090 Vienna, Austria
Address correspondence to Anna Lobell, Pharmacia & Upjohn, P12:4, 112 87 Stockholm, Sweden. Phone: 46-8-6958303; Fax: 46-8-6185882; E-mail: [email protected]
Received:
December 16 1997
Revision Received:
February 20 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1998
J Exp Med (1998) 187 (9): 1543–1548.
Article history
Received:
December 16 1997
Revision Received:
February 20 1998
Citation
Anna Lobell, Robert Weissert, Maria K. Storch, Cecilia Svanholm, Katrien L. de Graaf, Hans Lassmann, Roland Andersson, Tomas Olsson, Hans Wigzell; Vaccination with DNA Encoding an Immunodominant Myelin Basic Protein Peptide Targeted to Fc of Immunoglobulin G Suppresses Experimental Autoimmune Encephalomyelitis . J Exp Med 4 May 1998; 187 (9): 1543–1548. doi: https://doi.org/10.1084/jem.187.9.1543
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