Evidence is growing for both humoral and cellular immune recognition of human tumor antigens. Antibodies with specificity for antigens initially recognized by cytotoxic T lymphocytes (CTLs), e.g., MAGE and tyrosinase, have been detected in melanoma patient sera, and CTLs with specificity for NY-ESO-1, a cancer-testis (CT) antigen initially identified by autologous antibody, have recently been identified. To establish a screening system for the humoral response to autoimmunogenic tumor antigens, an enzyme-linked immunosorbent assay (ELISA) was developed using recombinant NY-ESO-1, MAGE-1, MAGE-3, SSX2, Melan-A, and tyrosinase proteins. A survey of sera from 234 cancer patients showed antibodies to NY-ESO-1 in 19 patients, to MAGE-1 in 3, to MAGE-3 in 2, and to SSX2 in 1 patient. No reactivity to these antigens was found in sera from 70 normal individuals. The frequency of NY-ESO-1 antibody was 9.4% in melanoma patients and 12.5% in ovarian cancer patients. Comparison of tumor NY-ESO-1 phenotype and NY-ESO-1 antibody response in 62 stage IV melanoma patients showed that all patients with NY-ESO-1+ antibody had NY-ESO-1+ tumors, and no patients with NY-ESO-1− tumors had NY-ESO-1 antibody. As the proportion of melanomas expressing NY-ESO-1 is 20–40% and only patients with NY-ESO-1+ tumors have antibody, this would suggest that a high percentage of patients with NY-ESO-1+ tumors develop an antibody response to NY-ESO-1.
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20 April 1998
Brief Definitive Report|
April 20 1998
A Survey of the Humoral Immune Response of Cancer Patients to a Panel of Human Tumor Antigens
Elisabeth Stockert,
Elisabeth Stockert
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
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Elke Jäger,
Elke Jäger
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
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Yao-Tseng Chen,
Yao-Tseng Chen
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
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Matthew J. Scanlan,
Matthew J. Scanlan
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
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Ivan Gout,
Ivan Gout
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
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Julia Karbach,
Julia Karbach
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
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Michael Arand,
Michael Arand
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
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Alexander Knuth,
Alexander Knuth
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
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Lloyd J. Old
Lloyd J. Old
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
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Elisabeth Stockert
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
Elke Jäger
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
Yao-Tseng Chen
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
Matthew J. Scanlan
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
Ivan Gout
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
Julia Karbach
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
Michael Arand
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
Alexander Knuth
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
Lloyd J. Old
From the *Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021; ‡II. Medizinische Klinik, Hämatologie–Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany; the §Cornell University Medical College, New York 10021; ‖Ludwig Institute for Cancer Research, London Branch at University College London School of Medicine, W1P 8BT London, United Kingdom; and the ¶Institut für Toxikologie, Johannes Gutenberg Universität, 55131 Mainz, Germany
Address correspondence to Elisabeth Stockert, Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, 1275 York Ave., New York, NY 10021. Phone: 212-639-7542; Fax: 212-717-3100; E-mail: [email protected]
This work was partially funded by National Institutes of Health grant CA-68024.
Received:
November 10 1997
Revision Received:
January 30 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1998
J Exp Med (1998) 187 (8): 1349–1354.
Article history
Received:
November 10 1997
Revision Received:
January 30 1998
Citation
Elisabeth Stockert, Elke Jäger, Yao-Tseng Chen, Matthew J. Scanlan, Ivan Gout, Julia Karbach, Michael Arand, Alexander Knuth, Lloyd J. Old; A Survey of the Humoral Immune Response of Cancer Patients to a Panel of Human Tumor Antigens . J Exp Med 20 April 1998; 187 (8): 1349–1354. doi: https://doi.org/10.1084/jem.187.8.1349
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