Activation of T and natural killer (NK) cells leads to the tyrosine phosphorylation of pp36 and to its association with several signaling molecules, including phospholipase Cγ-1 and Grb2. Microsequencing of peptides derived from purified rat pp36 protein led to the cloning, in rat and man, of cDNA encoding a T- and NK cell–specific protein with several putative Src homology 2 domain–binding motifs. A rabbit antiserum directed against a peptide sequence from the cloned rat molecule recognized tyrosine phosphorylated pp36 from pervanadate-treated rat thymocytes. When expressed in 293T human fibroblast cells and tyrosine-phosphorylated, pp36 associated with phospholipase Cγ-1 and Grb2. Studies with GST–Grb2 fusion proteins demonstrated that the association was specific for the Src homology 2 domain of Grb-2. Molecular cloning of the gene encoding pp36 should facilitate studies examining the role of this adaptor protein in proximal signaling events during T and NK cell activation.
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6 April 1998
Brief Definitive Report|
April 06 1998
Molecular Cloning of the cDNA Encoding pp36, a Tyrosine-phosphorylated Adaptor Protein Selectively Expressed by T Cells and Natural Killer Cells
John R. Weber,
John R. Weber
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
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Sigurd Ørstavik,
Sigurd Ørstavik
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
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Knut Martin Torgersen,
Knut Martin Torgersen
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
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Niels Christian Danbolt,
Niels Christian Danbolt
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
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Siri F. Berg,
Siri F. Berg
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
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James C. Ryan,
James C. Ryan
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
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Kjetil Taskén,
Kjetil Taskén
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
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John B. Imboden,
John B. Imboden
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
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John T. Vaage
John T. Vaage
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
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John R. Weber
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
Sigurd Ørstavik
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
Knut Martin Torgersen
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
Niels Christian Danbolt
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
Siri F. Berg
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
James C. Ryan
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
Kjetil Taskén
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
John B. Imboden
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
John T. Vaage
From the *Department of Medicine and Rosalind Russell Arthritis Center, University of California, San Francisco, California 94143; the ‡Institute of Medical Biochemistry and §Department of Anatomy, University of Oslo, N-0317 Oslo, Norway; and the ‖Veterans Administration Medical Center, University of California, San Francisco, California 94122
Address correspondence to John B. Imboden, Box 0868, Department of Medicine, University of California, San Francisco, CA 94143. Phone: 415-206-6641; Fax: 415-648-8425; E-mail: [email protected]
Note added in proof. Zhang et al. recently reported the cloning of human and mouse pp36. Cell. 92:83.
Received:
January 26 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1998
J Exp Med (1998) 187 (7): 1157–1161.
Article history
Received:
January 26 1998
Citation
John R. Weber, Sigurd Ørstavik, Knut Martin Torgersen, Niels Christian Danbolt, Siri F. Berg, James C. Ryan, Kjetil Taskén, John B. Imboden, John T. Vaage; Molecular Cloning of the cDNA Encoding pp36, a Tyrosine-phosphorylated Adaptor Protein Selectively Expressed by T Cells and Natural Killer Cells . J Exp Med 6 April 1998; 187 (7): 1157–1161. doi: https://doi.org/10.1084/jem.187.7.1157
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