The effect of preexistent virus-neutralizing antibodies on the active induction of antiviral T cell responses was studied in two model infections in mice. Against the noncytopathic lymphocytic choriomeningitis virus (LCMV), pretreatment with neutralizing antibodies conferred immediate protection against systemic virus spread and controlled the virus below detectable levels. However, presence of protective antibody serum titers did not impair induction of antiviral cytotoxic T lymphocyte (CTL) responses after infection with 102 PFU of LCMV. These CTLs efficiently protected mice independent of antibodies against challenge with LCMV–glycoprotein recombinant vaccinia virus; they also protected against otherwise lethal lymphocytic choriomeningitis caused by intracerebral challenge with LCMV-WE, whereas transfused antibodies alone did not protect, and in some cases even enhanced, lethal lymphocytic choriomeningitis. Against the cytopathic vesicular stomatitis virus (VSV), specific CTLs and Th cells were induced in the presence of high titers of VSV-neutralizing antibodies after infection with 106 PFU of VSV, but not at lower virus doses. Taken together, preexistent protective antibody titers controlled infection but did not impair induction of protective T cell immunity. This is particularly relevant for noncytopathic virus infections since both virus-neutralizing antibodies and CTLs are essential for continuous virus control. Therefore, to vaccinate against such viruses parallel or sequential passive and active immunization may be a suitable vaccination strategy to combine advantages of both virus-neutralizing antibodies and CTLs.
Skip Nav Destination
Article navigation
16 February 1998
Brief Definitive Report|
February 16 1998
Induction of Protective Cytotoxic T Cell Responses in the Presence of High Titers of Virus-neutralizing Antibodies: Implications for Passive and Active Immunization
Peter Seiler,
Peter Seiler
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Search for other works by this author on:
Marie-Anne Bründler,
Marie-Anne Bründler
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Search for other works by this author on:
Christine Zimmermann,
Christine Zimmermann
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Search for other works by this author on:
Doris Weibel,
Doris Weibel
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Search for other works by this author on:
Michael Bruns,
Michael Bruns
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Search for other works by this author on:
Hans Hengartner,
Hans Hengartner
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Search for other works by this author on:
Rolf M. Zinkernagel
Rolf M. Zinkernagel
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Search for other works by this author on:
Peter Seiler
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Marie-Anne Bründler
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Christine Zimmermann
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Doris Weibel
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Michael Bruns
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Hans Hengartner
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
Rolf M. Zinkernagel
From the *Institute of Experimental Immunology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and the ‡Heinrich Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany
This work was supported by Swiss National Science Foundation grants 31-32195.91, 31-32179.91, 31-50884.97, and 31-50900.97.
Address correspondence to Peter Seiler, Institute of Experimental Immunology, Department of Pathology, University of Zürich, Schmelzbergstrasse 12, CH 8091 Zürich, Switzerland. Phone: 41-1-255-29-89; Fax: 41-1-255-44-20.
Marie-Anne Bründler's current address is Division de pathologie clinique, centre medical universitaire (C.M.U.), rue Michel-Servet 1, 1211 Geneve 4, Switzerland.
Christine Zimmermann's present address is Institute for Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg, Hermann-Herder-Str. 11, D-79104 Freiburg, Germany.
Peter Seiler and Marie-Anne Bründler contributed equally to this work.
Received:
October 04 1997
Revision Received:
December 01 1997
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1998
J Exp Med (1998) 187 (4): 649–654.
Article history
Received:
October 04 1997
Revision Received:
December 01 1997
Citation
Peter Seiler, Marie-Anne Bründler, Christine Zimmermann, Doris Weibel, Michael Bruns, Hans Hengartner, Rolf M. Zinkernagel; Induction of Protective Cytotoxic T Cell Responses in the Presence of High Titers of Virus-neutralizing Antibodies: Implications for Passive and Active Immunization . J Exp Med 16 February 1998; 187 (4): 649–654. doi: https://doi.org/10.1084/jem.187.4.649
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
