Dendritic cells initiate immune responses by ferrying antigen from the tissues to the lymphoid organs for presentation to lymphocytes. Little is known about the molecular mechanisms underlying this migratory behavior. We have identified a chemokine receptor which appears to be selectively expressed in human dendritic cells derived from CD34+ cord blood precursors, but not in dendritic cells derived from peripheral blood monocytes. When stably expressed as a recombinant protein in a variety of host cell backgrounds, the receptor shows a strong interaction with only one chemokine among 25 tested: the recently reported CC chemokine macrophage inflammatory protein 3α. Thus, we have designated this receptor as the CC chemokine receptor 6. The cloning and characterization of a dendritic cell CC chemokine receptor suggests a role for chemokines in the control of the migration of dendritic cells and the regulation of dendritic cell function in immunity and infection.
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15 September 1997
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September 15 1997
CCR6, a CC Chemokine Receptor that Interacts with Macrophage Inflammatory Protein 3α and Is Highly Expressed in Human Dendritic Cells
David R. Greaves,
David R. Greaves
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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Wei Wang,
Wei Wang
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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Daniel J. Dairaghi,
Daniel J. Dairaghi
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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Marie Caroline Dieu,
Marie Caroline Dieu
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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Blandine de Saint-Vis,
Blandine de Saint-Vis
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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Karin Franz-Bacon,
Karin Franz-Bacon
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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Devora Rossi,
Devora Rossi
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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Christophe Caux,
Christophe Caux
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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Terrill McClanahan,
Terrill McClanahan
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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Siamon Gordon,
Siamon Gordon
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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Albert Zlotnik,
Albert Zlotnik
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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Thomas J. Schall
Thomas J. Schall
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
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David R. Greaves
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Wei Wang
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Daniel J. Dairaghi
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Marie Caroline Dieu
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Blandine de Saint-Vis
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Karin Franz-Bacon
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Devora Rossi
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Christophe Caux
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Terrill McClanahan
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Siamon Gordon
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Albert Zlotnik
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Thomas J. Schall
From the *Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; ‡Schering-Plough Laboratory for Immunological Research, Dardilly 69571, France; §Molecular Medicine Research Institute, Mountain View, California 94043; and ‖The DNAX Research Institute, Palo Alto, California 94304
Address correspondence to Thomas J. Schall, Molecular Medicine Research Institute, 325 E. Middlefield Rd., Mountain View, CA 94043. Phone: 415-237-7442; FAX: 415-237-7455; E-mail: [email protected]
DNAX is supported by Schering-Plough; work in the laboratory of S. Gordon is supported by the United Kingdom Medical Research Council; D.R. Greaves is supported by Glaxo Wellcome.
1
Abbreviations used in this paper: CCR, a receptor for CC chemokines; CHO, Chinese hamster ovary; CXCR, a receptor for CXC chemokines; DC, dendritic cell; HEK293, human embryonic kidney 293; IC2, second intracellular loop; LARC, liver activation–related chemokine; MIP, macrophage inflammatory protein; mRNA, messenger RNA; ORF, open reading frame; TM, transmembrane.
D.R. Greaves and W. Wang contributed equally to this study.
Received:
April 24 1997
Revision Received:
June 24 1997
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1997
J Exp Med (1997) 186 (6): 837–844.
Article history
Received:
April 24 1997
Revision Received:
June 24 1997
Citation
David R. Greaves, Wei Wang, Daniel J. Dairaghi, Marie Caroline Dieu, Blandine de Saint-Vis, Karin Franz-Bacon, Devora Rossi, Christophe Caux, Terrill McClanahan, Siamon Gordon, Albert Zlotnik, Thomas J. Schall; CCR6, a CC Chemokine Receptor that Interacts with Macrophage Inflammatory Protein 3α and Is Highly Expressed in Human Dendritic Cells . J Exp Med 15 September 1997; 186 (6): 837–844. doi: https://doi.org/10.1084/jem.186.6.837
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