CD3-ζ/η–deficient mice have small thymuses containing cells that show a profound reduction in the surface levels of T cell receptors and terminate their differentiation at the CD4+CD8+ stage. Rather unexpectedly, CD3− or very low single positive T cells accumulate over time in the spleen and lymph nodes of CD3-ζ/η–deficient mice after a process dependent on MHC expression. Fusion of these peripheral T cells with a CD3-ζ–positive derivative of the BW5147 TCR-α−/β− thymoma resulted in hybridomas that do express an heterogeneous set of T cell receptor α/β dimers at their surface and at density comparable to those found in hybridomas derived from wild-type peripheral T cells. We have investigated the specificities of these T cell receptors using spleen cells from congenic and mutant mouse strains, and showed that the majority of them readily recognized self-MHC class I or class II molecules. These results demonstrate that by increasing the density and/or output of the T cell receptors expressed in peripheral T cells, one can confer them with the capacity to respond to normal density of self-MHC molecules.
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17 February 1997
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February 17 1997
The Single Positive T Cells Found in CD3-ζ/η−/− Mice Overtly React with Self–Major Histocompatibility Complex Molecules upon Restoration of Normal Surface Density of T Cell Receptor–CD3 Complex
Shih-Yao Lin,
Shih-Yao Lin
From the Centre d'Immunologie Institut National de la Santè et de la Recherche Mèdicale–Centre National de Recherche Scientifique de Marseille-Luminy, 13288 Marseille Cedex 9, France
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Laurence Ardouin,
Laurence Ardouin
From the Centre d'Immunologie Institut National de la Santè et de la Recherche Mèdicale–Centre National de Recherche Scientifique de Marseille-Luminy, 13288 Marseille Cedex 9, France
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Anne Gillet,
Anne Gillet
From the Centre d'Immunologie Institut National de la Santè et de la Recherche Mèdicale–Centre National de Recherche Scientifique de Marseille-Luminy, 13288 Marseille Cedex 9, France
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Marie Malissen,
Marie Malissen
From the Centre d'Immunologie Institut National de la Santè et de la Recherche Mèdicale–Centre National de Recherche Scientifique de Marseille-Luminy, 13288 Marseille Cedex 9, France
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Bernard Malissen
Bernard Malissen
From the Centre d'Immunologie Institut National de la Santè et de la Recherche Mèdicale–Centre National de Recherche Scientifique de Marseille-Luminy, 13288 Marseille Cedex 9, France
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Shih-Yao Lin
From the Centre d'Immunologie Institut National de la Santè et de la Recherche Mèdicale–Centre National de Recherche Scientifique de Marseille-Luminy, 13288 Marseille Cedex 9, France
Laurence Ardouin
From the Centre d'Immunologie Institut National de la Santè et de la Recherche Mèdicale–Centre National de Recherche Scientifique de Marseille-Luminy, 13288 Marseille Cedex 9, France
Anne Gillet
From the Centre d'Immunologie Institut National de la Santè et de la Recherche Mèdicale–Centre National de Recherche Scientifique de Marseille-Luminy, 13288 Marseille Cedex 9, France
Marie Malissen
From the Centre d'Immunologie Institut National de la Santè et de la Recherche Mèdicale–Centre National de Recherche Scientifique de Marseille-Luminy, 13288 Marseille Cedex 9, France
Bernard Malissen
From the Centre d'Immunologie Institut National de la Santè et de la Recherche Mèdicale–Centre National de Recherche Scientifique de Marseille-Luminy, 13288 Marseille Cedex 9, France
Address correspondence to Bernard Malissen, Centre d'Immunologie INSERM-CNRS de MarseilleLuminy, Case 906, 13288 Marseille Cedex 9, France.
Received:
September 19 1996
Revision Received:
December 02 1996
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1997
J Exp Med (1997) 185 (4): 707–716.
Article history
Received:
September 19 1996
Revision Received:
December 02 1996
Citation
Shih-Yao Lin, Laurence Ardouin, Anne Gillet, Marie Malissen, Bernard Malissen; The Single Positive T Cells Found in CD3-ζ/η−/− Mice Overtly React with Self–Major Histocompatibility Complex Molecules upon Restoration of Normal Surface Density of T Cell Receptor–CD3 Complex. J Exp Med 17 February 1997; 185 (4): 707–716. doi: https://doi.org/10.1084/jem.185.4.707
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