Adhesion of monocytes to the endothelium in lesion-prone areas is one of the earliest events in fatty streak formation leading to atherogenesis. The molecular basis of increased monocyte adhesion is not fully characterized. We have identified a novel vascular monocyte adhesion-associated protein, VMAP-1, that plays a role in adhesion of monocytes to activated endothelium. Originally selected for its ability to block binding of a mouse monocyte-like cell line (WEHI78/24) to cytokine- or LPS-stimulated cultured mouse endothelial cells in vitro, antiVMAP-1 mAb LM151 cross-reacts with rabbit endothelium and blocks binding of human monocytes to cultured rabbit aortic endothelial cells stimulated with minimally modified low density lipoprotein, thought to be a physiologically relevant atherogenic stimulus. Most importantly, LM151 prevents adhesion of normal monocytes and monocytoid cells to intact aortic endothelium from cholesterol-fed rabbits in an ex vivo assay. VMAP-1 is a 50-kD protein. Immunohistology of vessels reveals focal constitutive expression in aorta and other large vessels. VMAP-1 is thus a novel vascular adhesion-associated protein that appears to play a critical role in monocyte adhesion to aortic endothelial cells in atherogenesis in vivo.
Skip Nav Destination
Article navigation
16 June 1997
Article|
June 16 1997
Novel Vascular Molecule Involved in Monocyte Adhesion to Aortic Endothelium in Models of Atherogenesis
Leslie M. McEvoy,
Leslie M. McEvoy
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
Search for other works by this author on:
Hailing Sun,
Hailing Sun
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
Search for other works by this author on:
Philip S. Tsao,
Philip S. Tsao
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
Search for other works by this author on:
John P. Cooke,
John P. Cooke
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
Search for other works by this author on:
Judith A. Berliner,
Judith A. Berliner
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
Search for other works by this author on:
Eugene C. Butcher
Eugene C. Butcher
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
Search for other works by this author on:
Leslie M. McEvoy
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
Hailing Sun
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
Philip S. Tsao
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
John P. Cooke
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
Judith A. Berliner
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
Eugene C. Butcher
From the *Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, and Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, California 94304; ‡Department of Cardiovascular Medicine, Stanford University, Stanford, California 94305; §Department of Pathology, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California 90024
Address correspondence to Leslie M. McEvoy, Department of Pathology, L235, Stanford University, Stanford, CA 94305.
L.M. McEvoy was a Senior Fellow of the American Heart Association, California Division, and the National Multiple Sclerosis Society during part of this work. H. Sun is supported by Public Health Service grant No. CAO9302 and a predoctoral award from the National Cancer Institute. P.S. Tsao was the recipient of a National Service Research Award. J.P. Cooke was a recipient of the Vascular Academic Award from the National Heart, Lung, and Blood Institute. This work was supported by grants from the National Institutes of Health and the Core Facilities of the Stanford Digestive Disease Center under DK38707.
1Abbreviations used in this paper: GPI, glycosylphosphatidylinositol; HDL, high density lipoprotein; HEV, high endothelial venules; ICAM, intracellular adhesion molecule; MM-LDL, minimally modified LDL; PI-PLC, phosphatidylinositol-specific phospholipase; MAdCAM, mucosal addressin cell adhesion molecule; PP, Peyer's patch; RAEC, rabbit aortic endothelial cells; RT, room temperature; VCAM, vascular cell adhesion molecule; VMAP-1, vascular monocyte adhesion-associated protein 1.
Received:
January 15 1997
Revision Received:
March 31 1997
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1997
J Exp Med (1997) 185 (12): 2069–2077.
Article history
Received:
January 15 1997
Revision Received:
March 31 1997
Citation
Leslie M. McEvoy, Hailing Sun, Philip S. Tsao, John P. Cooke, Judith A. Berliner, Eugene C. Butcher; Novel Vascular Molecule Involved in Monocyte Adhesion to Aortic Endothelium in Models of Atherogenesis. J Exp Med 16 June 1997; 185 (12): 2069–2077. doi: https://doi.org/10.1084/jem.185.12.2069
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
