Inhalation of antigen in immunized mice induces an infiltration of eosinophils into the airways and increased bronchial hyperreactivity as are observed in human asthma. We employed a model of late-phase allergic pulmonary inflammation in mice to address the role of leukotrienes (LT) in mediating airway eosinophilia and hyperreactivity to methacholine. Allergen intranasal challenge in OVA-sensitized mice induced LTB4 and LTC4 release into the airspace, widespread mucus occlusion of the airways, leukocytic infiltration of the airway tissue and broncho-alveolar lavage fluid that was predominantly eosinophils, and bronchial hyperreactivity to methacholine. Specific inhibitors of 5-lipoxygenase and 5-lipoxygenase-activating protein (FLAP) blocked airway mucus release and infiltration by eosinophils indicating a key role for leukotrienes in these features of allergic pulmonary inflammation. The role of leukotrienes or eosinophils in mediating airway hyperresponsiveness to aeroallergen could not be established, however, in this murine model.
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1 October 1996
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October 01 1996
The importance of leukotrienes in airway inflammation in a mouse model of asthma.
W R Henderson, Jr,
W R Henderson, Jr
Department of Medicine, University of Washington, Seattle 98195, USA.
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D B Lewis,
D B Lewis
Department of Medicine, University of Washington, Seattle 98195, USA.
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R K Albert,
R K Albert
Department of Medicine, University of Washington, Seattle 98195, USA.
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Y Zhang,
Y Zhang
Department of Medicine, University of Washington, Seattle 98195, USA.
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W J Lamm,
W J Lamm
Department of Medicine, University of Washington, Seattle 98195, USA.
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G K Chiang,
G K Chiang
Department of Medicine, University of Washington, Seattle 98195, USA.
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F Jones,
F Jones
Department of Medicine, University of Washington, Seattle 98195, USA.
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P Eriksen,
P Eriksen
Department of Medicine, University of Washington, Seattle 98195, USA.
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Y T Tien,
Y T Tien
Department of Medicine, University of Washington, Seattle 98195, USA.
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M Jonas,
M Jonas
Department of Medicine, University of Washington, Seattle 98195, USA.
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E Y Chi
E Y Chi
Department of Medicine, University of Washington, Seattle 98195, USA.
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W R Henderson, Jr
Department of Medicine, University of Washington, Seattle 98195, USA.
D B Lewis
Department of Medicine, University of Washington, Seattle 98195, USA.
R K Albert
Department of Medicine, University of Washington, Seattle 98195, USA.
Y Zhang
Department of Medicine, University of Washington, Seattle 98195, USA.
W J Lamm
Department of Medicine, University of Washington, Seattle 98195, USA.
G K Chiang
Department of Medicine, University of Washington, Seattle 98195, USA.
F Jones
Department of Medicine, University of Washington, Seattle 98195, USA.
P Eriksen
Department of Medicine, University of Washington, Seattle 98195, USA.
Y T Tien
Department of Medicine, University of Washington, Seattle 98195, USA.
M Jonas
Department of Medicine, University of Washington, Seattle 98195, USA.
E Y Chi
Department of Medicine, University of Washington, Seattle 98195, USA.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1996) 184 (4): 1483–1494.
Citation
W R Henderson, D B Lewis, R K Albert, Y Zhang, W J Lamm, G K Chiang, F Jones, P Eriksen, Y T Tien, M Jonas, E Y Chi; The importance of leukotrienes in airway inflammation in a mouse model of asthma.. J Exp Med 1 October 1996; 184 (4): 1483–1494. doi: https://doi.org/10.1084/jem.184.4.1483
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