Developmental activation of VDJ recombination at the T cell receptor (TCR) delta locus is controlled by an intronic transcriptional enhancer (E delta). Transcriptional activation by E delta is dependent on c-Myb. To determine whether c-Myb plays a role in the activation of TCR-delta gene rearrangement, we compared VDJ recombination in transgenic mice carrying two versions of a human TCR-delta gene minilocus recombination substrate. One includes a wild-type E delta, whereas the other carries an E delta with a mutation that abrogates c-Myb binding. We demonstrate that an intact Myb binding site is necessary for efficient rearrangement of the minilocus substrate, suggesting that c-Myb plays a crucial role in activating VDJ recombination at the endogenous TCR-delta locus.
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1 January 1996
Article|
January 01 1996
Regulation of T cell receptor delta gene rearrangement by c-Myb.
C Hernández-Munain,
C Hernández-Munain
Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.
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P Lauzurica,
P Lauzurica
Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.
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M S Krangel
M S Krangel
Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.
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C Hernández-Munain
Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.
P Lauzurica
Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.
M S Krangel
Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1996) 183 (1): 289–293.
Citation
C Hernández-Munain, P Lauzurica, M S Krangel; Regulation of T cell receptor delta gene rearrangement by c-Myb.. J Exp Med 1 January 1996; 183 (1): 289–293. doi: https://doi.org/10.1084/jem.183.1.289
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