One approach towards the development of a vaccine against malaria is to immunize against the parasite sexual stages that mediate transmission of the parasite from man to mosquito. Antibodies against these stages, ingested with the blood meal, inhibit the parasite development in the mosquito vector, constituting "transmission blocking immunity." Most epitopes involved in transmission-blocking immunity depend on the tertiary conformational structure of surface antigens. However, one of the transmission-blocking monoclonal antibodies we have raised against Plasmodium vivax reacts with a linear epitope on both asexual stages and gametes. This monoclonal antibody (A12) is capable of totally blocking development of the parasite in the mosquito host when tested in membrane feeding assays with gametocytes from P. vivax-infected patients. Immune screening of a P. vivax lambda gt11 genomic expression library with A12 led to the isolation of a clone to which was mapped the six-amino acid epitope recognized by A12. Antisera raised in mice against a 12-mer synthetic peptide containing this epitope coupled to bovine serum albumin not only had high titers of antipeptide antibodies as measured by enzyme-linked immunosorbent assay, but in addition recognized the same 24- and 57-kD parasite components as A12 on Western blots and reacted with the parasite by immunofluorescence. When tested in membrane feeding assays, these antibodies have significant suppressive effects on parasite development in the mosquito.
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1 January 1995
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January 01 1995
Transmission blocking immunity in Plasmodium vivax malaria: antibodies raised against a peptide block parasite development in the mosquito vector.
V A Snewin,
V A Snewin
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
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S Premawansa,
S Premawansa
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
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G M Kapilananda,
G M Kapilananda
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
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L Ratnayaka,
L Ratnayaka
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
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P V Udagama,
P V Udagama
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
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D M Mattei,
D M Mattei
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
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E Khouri,
E Khouri
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
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G Del Giudice,
G Del Giudice
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
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J S Peiris,
J S Peiris
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
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K N Mendis,
K N Mendis
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
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P H David
P H David
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
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V A Snewin
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
S Premawansa
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
G M Kapilananda
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
L Ratnayaka
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
P V Udagama
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
D M Mattei
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
E Khouri
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
G Del Giudice
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
J S Peiris
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
K N Mendis
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
P H David
Unité d'Immunoparasitologie, Centre National de la Recherche Scientifique URA361, Institut Pasteur, Paris, France.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1995) 181 (1): 357–362.
Citation
V A Snewin, S Premawansa, G M Kapilananda, L Ratnayaka, P V Udagama, D M Mattei, E Khouri, G Del Giudice, J S Peiris, K N Mendis, P H David; Transmission blocking immunity in Plasmodium vivax malaria: antibodies raised against a peptide block parasite development in the mosquito vector.. J Exp Med 1 January 1995; 181 (1): 357–362. doi: https://doi.org/10.1084/jem.181.1.357
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