Veto cell-mediated suppression of cytotoxic T lymphocyte (CTL) responses has been proposed as one mechanism by which self-tolerance is maintained in mature T cell populations. We have previously reported that murine bone marrow cells cultured in the presence of high-dose interleukin 2 (IL-2) (activated bone marrow cells [ABM]) mediate strong veto suppressor function. To examine mechanisms by which ABM may suppress precursor CTL (p-CTL) responses, we used p-CTL generated from spleen cells of transgenic mice expressing a T cell receptor specific for H-2 Ld. It was demonstrated that the cytotoxic response by these p-CTL after stimulation with irradiated H-2d/k spleen cells was suppressed by DBA/2 (H-2d) ABM, but not by B10.BR (H-2k) ABM or dm1 (Dd, Ld mutant) ABM. Flow cytometry analysis with propidium iodide staining revealed that these p-CTL were specifically deleted by incubation with H-2d ABM, but not with H-2k ABM. These data indicate that ABM veto cells kill p-CTL with specificity for antigens expressed on the surface of the ABM, and that the mechanism for veto cell activity of ABM is clonal deletion of p-CTL.
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1 March 1992
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March 01 1992
Clonal deletion of postthymic T cells: veto cells kill precursor cytotoxic T lymphocytes.
K Hiruma,
K Hiruma
Experimental Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.
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H Nakamura,
H Nakamura
Experimental Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.
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P A Henkart,
P A Henkart
Experimental Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.
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R E Gress
R E Gress
Experimental Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.
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K Hiruma
Experimental Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.
H Nakamura
Experimental Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.
P A Henkart
Experimental Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.
R E Gress
Experimental Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1992) 175 (3): 863–868.
Citation
K Hiruma, H Nakamura, P A Henkart, R E Gress; Clonal deletion of postthymic T cells: veto cells kill precursor cytotoxic T lymphocytes.. J Exp Med 1 March 1992; 175 (3): 863–868. doi: https://doi.org/10.1084/jem.175.3.863
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