T cell clones recognizing the sperm whale myoglobin (SpWMb) epitope 110-121 in association with H-2d major histocompatibility complex class II molecules display a very limited heterogeneity of T cell receptor (TCR) V beta usage in DBA/2 mice. All clones previously tested used the same V beta 8.2 gene segment and very restricted junctional regions. To investigate the significance of this observation in vivo, we immunized DBA/2 mice with the intact SpW Mb protein or peptide 110-121. Only the V beta 8+ T cells showed any significant response to the 110-121 epitope. The response to peptide 110-121 was then analyzed in mice which, either as a consequence of antibody depletion or through genetic deletion of TCR V beta genes, lacked V beta 8+ peripheral T cells. DBA/2 mice depleted of V beta 8+ T cells by antibody treatment responded poorly to the 110-121 peptide, and only at high antigen concentrations. In contrast, DBA/2V beta a mice (homozygous for a deletion of multiple V beta gene segments including the V beta 8 family) made a response at least as great as that made by DBA/2 mice, even though the DBA/2V beta a mice had a very restricted TCR V beta repertoire compared with DBA/2 mice. Mechanisms which might determine differences in the 110-121 specific response of DBA/2, DBA/2V beta a and F23.1-treated DBA/2 mice are discussed.
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1 July 1991
Article|
July 01 1991
The T cell receptor repertoire influences V beta element usage in response to myoglobin.
G Ruberti,
G Ruberti
Department of Medicine, Stanford University School of Medicine, California 94305.
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A Gaur,
A Gaur
Department of Medicine, Stanford University School of Medicine, California 94305.
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C G Fathman,
C G Fathman
Department of Medicine, Stanford University School of Medicine, California 94305.
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A M Livingstone
A M Livingstone
Department of Medicine, Stanford University School of Medicine, California 94305.
Search for other works by this author on:
G Ruberti
Department of Medicine, Stanford University School of Medicine, California 94305.
A Gaur
Department of Medicine, Stanford University School of Medicine, California 94305.
C G Fathman
Department of Medicine, Stanford University School of Medicine, California 94305.
A M Livingstone
Department of Medicine, Stanford University School of Medicine, California 94305.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1991) 174 (1): 83–92.
Citation
G Ruberti, A Gaur, C G Fathman, A M Livingstone; The T cell receptor repertoire influences V beta element usage in response to myoglobin.. J Exp Med 1 July 1991; 174 (1): 83–92. doi: https://doi.org/10.1084/jem.174.1.83
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