We have analyzed the phenotypic characteristics and IgH gene rearrangements in a panel of EBV-transformed B lineage cell lines from human fetal liver and bone marrow. Some lines contained only populations of immature, Ig- Be cells, while others contained mixed populations of mature and immature B cells. The majority of identifiable IgH rearrangements involved joining of the most JH-proximal D segment, DQ52, to various JH segments, implying that DQ52 is a preferred target for initial DJH rearrangements. Three other rearrangements involving VH-related sequences were also characterized. Two involved VHDJH joining using VH3 genes, although one of these had a very unusual DJH structure. The third consisted of inverted 3' signal sequences and flanking regions of a VH4 gene appended to a JH. The mechanisms by which the later rearrangement could have occurred and its potential physiological significance are discussed.
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1 April 1989
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April 01 1989
Early human IgH gene assembly in Epstein-Barr virus-transformed fetal B cell lines. Preferential utilization of the most JH-proximal D segment (DQ52) and two unusual VH-related rearrangements.
K G Nickerson,
K G Nickerson
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
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J Berman,
J Berman
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
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E Glickman,
E Glickman
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
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L Chess,
L Chess
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
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F W Alt
F W Alt
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
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K G Nickerson
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
J Berman
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
E Glickman
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
L Chess
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
F W Alt
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1989) 169 (4): 1391–1403.
Citation
K G Nickerson, J Berman, E Glickman, L Chess, F W Alt; Early human IgH gene assembly in Epstein-Barr virus-transformed fetal B cell lines. Preferential utilization of the most JH-proximal D segment (DQ52) and two unusual VH-related rearrangements.. J Exp Med 1 April 1989; 169 (4): 1391–1403. doi: https://doi.org/10.1084/jem.169.4.1391
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