Evidence was sought on the tissue distribution of Mlsa determinants, a class of cell-associated non-H-2 alloantigens that is highly immunogenic for unprimed T cells. Whereas normal CD4+ T cells and an Mlsa-reactive T hybridoma gave strong responses to Mlsa-positive stimulator populations containing Ig+ B cells, anti-Mlsa responses to B-depleted stimulators were almost undetectable. The B-depleted stimulators tested included Thy-1- spleen cells from mu-suppressed mice (mice treated with anti-mu antibody from birth) and J11d- preparations of spleen dendritic cells (DC) and peritoneal macrophages (M phi) from normal mice. Each of these populations was strongly immunogenic for allo-H-2-reactive T cells. The failure to detect Mlsa determinants on Ig- APC, i.e., M phi and DC, suggests that Mlsa determinants are not typical H-2-associated peptides. The data are more compatible with a model in which Mlsa determinants represent (or form part of) an integral cell membrane molecule expressed largely, and perhaps exclusively, on B cells. T cells might recognize these molecules only in native form, "processed" Mlsa determinants being nonimmunogenic. Consistent with this possibility, no evidence was found that Mlsa-negative B cells could absorb Mlsa determinants from Mlsa-positive B cells in a chimeric environment.
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1 January 1989
Article|
January 01 1989
Restricted tissue distribution of Mlsa determinants. Stimulation of Mlsa-reactive T cells by B cells but not by dendritic cells or macrophages.
S R Webb,
S R Webb
Research Institute of Scripps Clinic, La Jolla, California 92037.
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A Okamoto,
A Okamoto
Research Institute of Scripps Clinic, La Jolla, California 92037.
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Y Ron,
Y Ron
Research Institute of Scripps Clinic, La Jolla, California 92037.
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J Sprent
J Sprent
Research Institute of Scripps Clinic, La Jolla, California 92037.
Search for other works by this author on:
S R Webb
Research Institute of Scripps Clinic, La Jolla, California 92037.
A Okamoto
Research Institute of Scripps Clinic, La Jolla, California 92037.
Y Ron
Research Institute of Scripps Clinic, La Jolla, California 92037.
J Sprent
Research Institute of Scripps Clinic, La Jolla, California 92037.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1989) 169 (1): 1–12.
Citation
S R Webb, A Okamoto, Y Ron, J Sprent; Restricted tissue distribution of Mlsa determinants. Stimulation of Mlsa-reactive T cells by B cells but not by dendritic cells or macrophages.. J Exp Med 1 January 1989; 169 (1): 1–12. doi: https://doi.org/10.1084/jem.169.1.1
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