We have identified strain-specific antigens with Camp and St. Lucia strains of P. falciparum of Mr approximately 285,000 and approximately 260,000, respectively. These strain-specific antigens were metabolically labeled with radioactive amino acids, indicating that they were of parasite origin rather than altered host components. These proteins had the properties of a molecule exposed on the surface of infected erythrocytes (IE). First, the proteins are accessible to lactoperoxidase-catalyzed radioiodination of IE. Second, the radioiodinated proteins were cleaved by low concentrations of trypsin (0.1 microgram/ml). Third, these antigens were immunoprecipitated after addition of immune sera to intact IE. Fourth, the strain-specific immuno-precipitation of these proteins correlated with the capacity of immune sera to block cytoadherence of IE in a strain-specific fashion. Fifth, the strain-specific antigen had detergent solubility properties (i.e., insolubility in 1% Triton X-100, solubility in 5% sodium dodecyl sulfate) similar to the variant antigen of P. knowlesi, which has been proven to be a malarial protein exposed on the erythrocyte surface.
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1 June 1984
Article|
June 01 1984
Identification of a strain-specific malarial antigen exposed on the surface of Plasmodium falciparum-infected erythrocytes.
J H Leech
J W Barnwell
L H Miller
R J Howard
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1984) 159 (6): 1567–1575.
Citation
J H Leech, J W Barnwell, L H Miller, R J Howard; Identification of a strain-specific malarial antigen exposed on the surface of Plasmodium falciparum-infected erythrocytes.. J Exp Med 1 June 1984; 159 (6): 1567–1575. doi: https://doi.org/10.1084/jem.159.6.1567
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