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Journal Articles
The Journal of Cell Biology (2020) 219 (3): e201905228.
Published: 15 January 2020
Journal Articles
The Journal of Cell Biology (2020) 219 (2): e201907083.
Published: 13 January 2020
Includes: Supplementary data
Images
Adaptor interaction sites on the CHC NTD directly interact with GTSE1. (A) ...
Published: 13 January 2020
Figure 1. Adaptor interaction sites on the CHC NTD directly interact with GTSE1. (A) Immunoblots of cell lysates (input) and IPs of GTSE1 and CHC in U2OS and GTSE1 knockout (GTSE1 KO ) cells. Immunoblots with anti-GTSE1 or anti-CHC antibody. (B) Immunoblot of cell lysates (input) and IPs of GT... More
Images
Clathrin recruits GTSE1 to the spindle. (A)  Immunofluorescence images of U...
Published: 13 January 2020
Figure 2. Clathrin recruits GTSE1 to the spindle. (A) Immunofluorescence images of U2OS cells stably expressing an RNAi-resistant GTSE1-GFP BAC transgene, stained with antibodies against GFP and α-tubulin. Cells were transfected with control (Ctrl) or CHC siRNA (66 h). All cells were concurrentl... More
Images
RNAi depletion efficiencies. (A)  Immunoblot on the cell lysate of U2OS cel...
Published: 13 January 2020
Figure S1. RNAi depletion efficiencies. (A) Immunoblot on the cell lysate of U2OS cells expressing an siRNA-resistant GTSE1-GFP BAC transgene and transfected with control (Ctrl) or CHC siRNA for 66 h. Cells were concomitantly transfected with GTSE1 siRNA to deplete endogenous GTSE1. Immunoblots ... More
Images
Multiple CBMs on GTSE1 and adaptor interaction sites on CHC are required fo...
Published: 13 January 2020
Figure 3. Multiple CBMs on GTSE1 and adaptor interaction sites on CHC are required for the GTSE1–CHC interaction. (A) Sequence alignment of GTSE1 C-terminus (aa 661–727 of human GTSE1). Putative clathrin-binding motifs are in green; divergent motifs are italicized. Mutations in the “5xLID” mutan... More
Images
CBMs on the GTSE1 C-terminus interact with adaptor binding sites on the CHC...
Published: 13 January 2020
Figure S2. CBMs on the GTSE1 C-terminus interact with adaptor binding sites on the CHC NTD. (A) GST-pulldown analysis of the interaction between GST-CHC(1–364) and GTSE1 fragments fused to a His-tag (GTSE1-His). Numbers indicate amino acids present in the fragments. Immunoblot is with anti-His a... More
Images
Sequence alignments of GTSE1 and TACC3. (A)  MUSCLE alignment of the N-term...
Published: 13 January 2020
Figure S3. Sequence alignments of GTSE1 and TACC3. (A) MUSCLE alignment of the N-terminus (amino acids 1–143 from human GTSE1) and C-terminus (amino acids 679–739 from human GTSE1) of potential GTSE1 homologues. Alignment was performed on full-length genes from the indicated organisms. A conserv... More
Images
The CHC/TACC3 complex recruits GTSE1 to the spindle to promote chromosome c...
Published: 13 January 2020
Figure 4. The CHC/TACC3 complex recruits GTSE1 to the spindle to promote chromosome congression. (A) Western blots of U2OS clonal lines expressing RNAi-resistant GTSE1-GFP (GTSE1 WT ) or GTSE1 5xLID -GFP (GTSE1 5xLID ) from BAC transgenes. Cells were transfected with control (Ctrl) or GTSE1 siRN... More
Images
The CHC–GTSE1 interaction recruits GTSE1 to the CHC/TACC3 complex and the s...
Published: 13 January 2020
Figure S4. The CHC–GTSE1 interaction recruits GTSE1 to the CHC/TACC3 complex and the spindle but does not control TACC3 spindle localization. (A) Immunoblot on mitotic lysates of GTSE1 WT and GTSE1 5xLID cells (Input) and IPs of the GFP transgene (GFP IP). MLN8054 was used to inhibit Aurora A.... More
Images
Loss of the CHC–GTSE1 interaction impacts spindle shape but not k-fiber sta...
Published: 13 January 2020
Figure 5. Loss of the CHC–GTSE1 interaction impacts spindle shape but not k-fiber stability. (A) Immunofluorescence images of mitotic spindles (α-tubulin staining) from U2OS, GTSE1 WT , and GTSE1 5xLID cells, transfected with control (Ctrl) or GTSE1 siRNA. (B–D) The ratio of spindle width ove... More
Images
The CHC–GTSE1 interaction is required for stabilization of nonkinetochore M...
Published: 13 January 2020
Figure 6. The CHC–GTSE1 interaction is required for stabilization of nonkinetochore MTs. (A) Immunofluorescence images showing STLC-induced monopolar spindles in indicated cell lines and RNAi conditions. Dashed yellow lines indicate cell borders. (B and C) Mean growing MT length per cell (B) a... More
Images
Analysis of chromosome congression in GTSE1 5xLID  cells and quantification...
Published: 13 January 2020
Figure S5. Analysis of chromosome congression in GTSE1 5xLID cells and quantification of Aurora B kinase abundance and activity after GTSE1 depletion. (A) Immunoblot on asynchronous cell lysates of GTSE1 5xLID cells expressing a mCherry-β-tubulin BAC transgene and transfected with control (Ctr... More
Images
Astral MTs are destabilized upon disruption of the CHC-GTSE1 interaction. (...
Published: 13 January 2020
Figure 7. Astral MTs are destabilized upon disruption of the CHC-GTSE1 interaction. (A) Immunofluorescence images illustrating astral length and abundance (α-tubulin and EB1 staining) under labeled conditions. (B) Scheme illustrating the determination of “astral” EB1 comets and quantification ... More
Images
Clathrin promotes chromosome congression and timely mitosis by antagonizing...
Published: 13 January 2020
Figure 8. Clathrin promotes chromosome congression and timely mitosis by antagonizing MCAK activity via GTSE1 recruitment. (A) Immunoblots of cell lysates (input) and anti-GFP IPs of GTSE1-GFP or GTSE1 5xLID -GFP under conditions illustrated. Immunoblots with anti-GFP, anti-CHC, or anti-MCAK ant... More
Images
Model of the clathrin/TACC3 complex on a MT.  Schematic model of the clathr...
Published: 13 January 2020
Figure 9. Model of the clathrin/TACC3 complex on a MT. Schematic model of the clathrin/TACC3 complex on a MT based on studies to date. The Aurora A–dependent phosphorylation of S558 on TACC3 is represented as a P. TACC3 is also bound to the MT polymerase ch-TOG. The CHC NTD recruits GTSE1 to inh... More
Journal Articles
The Journal of Cell Biology (2020) 219 (2): e201912006.
Published: 13 January 2020
Images
AIS-located proteasomes degrade chloride transporters and drive GABA respon...
Published: 13 January 2020
Figure 1. AIS-located proteasomes degrade chloride transporters and drive GABA response reversal. Proteasomes associated with the adaptor Ecm29 (green) are transported to the AIS by kinesins along microtubules (gray). Here, they interact with ankyrin G (ankG; brown) and degrade NKCC1 chloride tr... More
Journal Articles
The Journal of Cell Biology (2020) 219 (3): e201906039.
Published: 08 January 2020
Includes: Supplementary data
Journal Articles
The Journal of Cell Biology (2020) 219 (2): e201904137.
Published: 08 January 2020
Includes: Supplementary data