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Journal Articles
The Journal of Cell Biology (2020) 219 (3): e201908142.
Published: 20 January 2020
Journal Articles
The Journal of Cell Biology (2020) 219 (2): e201911139.
Published: 20 January 2020
Images
Model for the role of NuMA1 in AIS assembly.  (a)  In early development, DC...
Published: 20 January 2020
Figure 1. Model for the role of NuMA1 in AIS assembly. (a) In early development, DCX and Lis1 facilitate endocytosis of NF186, thus inhibiting the clustering of Neurofascin on plasma membrane. (b) During AIS assembly, NuMA1 inhibits the interaction of DCX and Lis1, leading to inhibition of en... More
Images
Recruitment of Aux1 and GAK to clathrin-coated vesicles in genome-edited ce...
Published: 20 January 2020
Figure 1. Recruitment of Aux1 and GAK to clathrin-coated vesicles in genome-edited cells. (A) CRISPR/Cas9 gene editing strategy used to incorporate EGFP at the N-terminus of Aux1 or GAK. The target sequence at the genomic locus of gene DNAJC6 (Aux1) recognized by the sgRNA, the protospacer adj... More
Images
Gene editing of SUM159 cells to express CLTA-TagRFP and EGFP-Aux1 or CLTA-T...
Published: 20 January 2020
Figure S1. Gene editing of SUM159 cells to express CLTA-TagRFP and EGFP-Aux1 or CLTA-TagRFP and EGFP-GAK. (A) CRISPR/Cas9 gene editing strategy used to incorporate EGFP at the N-terminus of Aux1 or GAK. The resulting DNA sequences, including the short linker between the C-terminus of EGFP and N... More
Images
Temporal and spatial distributions of Aux1 and GAK. (A and B)  Maximum-inte...
Published: 20 January 2020
Figure 2. Temporal and spatial distributions of Aux1 and GAK. (A and B) Maximum-intensity projections from a thin 2-µm optical section of a gene-edited cell expressing EGFP-Aux1 (A) or EGFP-GAK (B) recorded in 3D by lattice light-sheet microscopy. Scale bars, 10 µm. (C) Representative plot fro... More
Images
Recruitment of Aux1 and GAK to clathrin-coated vesicles in genome-edited ce...
Published: 20 January 2020
Figure S2. Recruitment of Aux1 and GAK to clathrin-coated vesicles in genome-edited cells. (A) Representative plots of single endocytic events (first three panels) and hotspots (last panel) showing fluorescence intensity traces for CLTA-TagRFP and EGFP-Aux1 (arbitrary units for CLTA; number of ... More
Images
Sequential bursts of Aux1 and GAK during uncoating of clathrin-coated vesic...
Published: 20 January 2020
Figure S3. Sequential bursts of Aux1 and GAK during uncoating of clathrin-coated vesicles at the plasma membrane and recruitment of GAK to the intracellular clathrin-containing carriers. (A) The TagRFP sequence was inserted into the GAK genomic locus of the EGFP-Aux1 +/+ cells to generate the d... More
Images
Recruitment of Aux1, GAK, and phosphoinositide sensors to clathrin-coated v...
Published: 20 January 2020
Figure 3. Recruitment of Aux1, GAK, and phosphoinositide sensors to clathrin-coated vesicles. (A–H) Bottom (adherent) surfaces of cells transiently expressing various combinations of Aux1, GAK, and Aux1-based PtdIns(3)P and PtdIns(4)P sensors imaged by TIRF microscopy every 0.5 s for 100 s. (A)... More
Images
Influence of PTEN-like domains on Aux1 and GAK recruitment. (A–L)  Bottom s...
Published: 20 January 2020
Figure 4. Influence of PTEN-like domains on Aux1 and GAK recruitment. (A–L) Bottom surfaces of gene-edited CLTA-TagRFP +/+ cells transiently expressing indicated EGFP-tagged constructs of Aux1 or GAK, imaged by TIRF microscopy every 1 s for 300 s. Each panel shows a schematic representation of ... More
Images
Effects on clathrin-mediated endocytosis of knockout or knockdown of GAK an...
Published: 20 January 2020
Figure S4. Effects on clathrin-mediated endocytosis of knockout or knockdown of GAK and knockdown of Aux1. (A) CRISPR/Cas9 gene editing strategy used to knock out GAK in cells gene edited for EGFP-Aux1 +/+ and CTLA-TagRFP +/+ . The double strand break (red triangles) induced by Cas9 resulted i... More
Images
Roles of the PTEN-like domain and clathrin-binding domain of auxilins in th...
Published: 20 January 2020
Figure S5. Roles of the PTEN-like domain and clathrin-binding domain of auxilins in the endocytic and secretory pathways. (A) AP2-TagRFP +/+ cells with or without GAK (AP2-TagRFP GAK-KO) treated with control siRNA or siRNA targeting Aux1 for 3 d (two sequential transfections), then subjected to... More
Images
Single-particle in vitro uncoating assay. (A)  Schematic representation of ...
Published: 20 January 2020
Figure 5. Single-particle in vitro uncoating assay. (A) Schematic representation of the assay. The intensities of fluorescence from labeled clathrin and lipid dyes incorporated into liposomes surrounded by clathrin/AP2 coats were monitored by TIRF microscopy. Synthetic clathrin/AP2-coated vesicl... More
Images
In vitro disassembly of clathrin/AP2 coats and sCCVs containing PtdIns(3)P ...
Published: 20 January 2020
Figure S6. In vitro disassembly of clathrin/AP2 coats and sCCVs containing PtdIns(3)P or PtdIns(4)P. (A) SDS-PAGE (and Coomassie blue staining) of the recombinant full-length Aux1, ΔPTEN-Aux1, and full-length GAK. Molecular weight markers are shown. For GAK and ΔPTEN-Aux1, impurities (of high el... More
Journal Articles
The Journal of Cell Biology (2020) 219 (3): e201908195.
Published: 17 January 2020
Includes: Supplementary data
Journal Articles
The Journal of Cell Biology (2020) 219 (3): e201905228.
Published: 15 January 2020
Journal Articles
The Journal of Cell Biology (2020) 219 (2): e201912006.
Published: 13 January 2020
Images
AIS-located proteasomes degrade chloride transporters and drive GABA respon...
Published: 13 January 2020
Figure 1. AIS-located proteasomes degrade chloride transporters and drive GABA response reversal. Proteasomes associated with the adaptor Ecm29 (green) are transported to the AIS by kinesins along microtubules (gray). Here, they interact with ankyrin G (ankG; brown) and degrade NKCC1 chloride tr... More
Journal Articles
The Journal of Cell Biology (2020) 219 (2): e201907083.
Published: 13 January 2020
Includes: Supplementary data
Images
Adaptor interaction sites on the CHC NTD directly interact with GTSE1. (A) ...
Published: 13 January 2020
Figure 1. Adaptor interaction sites on the CHC NTD directly interact with GTSE1. (A) Immunoblots of cell lysates (input) and IPs of GTSE1 and CHC in U2OS and GTSE1 knockout (GTSE1 KO ) cells. Immunoblots with anti-GTSE1 or anti-CHC antibody. (B) Immunoblot of cell lysates (input) and IPs of GT... More