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Journal Articles
The Journal of Cell Biology (2020) 219 (3): e201908195.
Published: 17 January 2020
Includes: Supplementary data
Images
Simulating the effect of a persistence gradient on the motion of a spherica...
Published: 17 January 2020
Figure 1. Simulating the effect of a persistence gradient on the motion of a spherical object similar in size to the nucleus. (A) Time frames from one 3D simulation of a gradient of persistence of actin-positive vesicles. The images correspond to Video 1. The nucleus is in blue, the actin-positi... More
Images
Testing the outcomes of simulations with or without any gradient of activit...
Published: 17 January 2020
Figure S1. Testing the outcomes of simulations with or without any gradient of activity. (A) Squared velocities of actin-positive vesicles as a function of the distance to the oocyte center during the centering phase. One red dot corresponds to the averaged square velocities of vesicles in the g... More
Images
Peripherally injected oil droplets are centered in oocytes in prophase I. (...
Published: 17 January 2020
Figure 2. Peripherally injected oil droplets are centered in oocytes in prophase I. (A) Transmitted light images of an oil droplet moving toward the center in a prophase I oocyte (observation at low temporal resolution, Δt = 20 min). Images correspond to Video 3. Frame interval displayed here is... More
Images
Example of an oscillatory motion of a droplet around the oocyte center. (A)...
Published: 17 January 2020
Figure S2. Example of an oscillatory motion of a droplet around the oocyte center. (A) Images in transmitted light of a droplet centered in prophase I. One image is shown every 140 min. Scale bar is 10 µm. The yellow arrow points at the oil droplet. (B) Distance of the droplet centroid to the ... More
Images
F-actin is responsible for the biased motion of oil droplets toward the ooc...
Published: 17 January 2020
Figure S3. F-actin is responsible for the biased motion of oil droplets toward the oocyte center. (A) Scheme explaining how the cumulative bias toward the oocyte center or cortex is measured. The black dots correspond to the centroid of objects. One track of an oil droplet centroid is presented ... More
Images
Oil droplets recapitulate nucleus behavior. (A)  Visualization of F-actin i...
Published: 17 January 2020
Figure 3. Oil droplets recapitulate nucleus behavior. (A) Visualization of F-actin in the region of the nucleus (upper panels) and of the droplet (lower panels) observed in the same oocyte in two different Z planes. F-actin is labeled with GFP-UtrCH, which is either highly expressed (left panels... More
Images
Prediction from simulations of object behavior as a function of object size...
Published: 17 January 2020
Figure 4. Prediction from simulations of object behavior as a function of object size. (A) Evolution of the distance of the nucleus centroid to the oocyte center in 30 different simulations for objects with a diameter >6 µm. One line corresponds to one simulation, and the color indicates the ... More
Images
Objects larger than a few micrometers are biased in their diffusion. (A)  I...
Published: 17 January 2020
Figure S4. Objects larger than a few micrometers are biased in their diffusion. (A) Images of fluorescent aggregates of beads (left panel) and an oil droplet in prophase I oocytes (right panel). Aggregates of beads correspond to objects with diameters between 100 nm and 2 µm. Oil droplets corres... More
Images
Oil droplets are also centered in oocytes undergoing meiosis I. (A)  Center...
Published: 17 January 2020
Figure 5. Oil droplets are also centered in oocytes undergoing meiosis I. (A) Centering of an oil droplet in an oocyte undergoing meiosis I. The images correspond to Video 7. One frame is shown every 140 min. The first frame corresponds to NEBD, a marker of meiosis resumption, and the last one t... More
Images
Experimental and simulated comparison of the efficiency of droplets centeri...
Published: 17 January 2020
Figure 6. Experimental and simulated comparison of the efficiency of droplets centering in prophase I versus meiosis I. (A) Radial velocities of droplet centroids as a function of the distance to the oocyte center for droplets centered during prophase I (red squares) and meiosis I (blue squares)... More
Images
Optical tweezers measurements.  Quantification of elastic and viscous modul...
Published: 17 January 2020
Figure S5. Optical tweezers measurements. Quantification of elastic and viscous moduli at 10 Hz for oocytes maintained in prophase I and oocytes undergoing meiosis I. For oocytes undergoing meiosis I, the measurements were taken at NEBD + 6 h. P values were calculated with a Kolmogorov-Smirnov s... More
Journal Articles
The Journal of Cell Biology (2020) 219 (3): e201905228.
Published: 15 January 2020
Journal Articles
The Journal of Cell Biology (2020) 219 (2): e201907083.
Published: 13 January 2020
Includes: Supplementary data
Images
Adaptor interaction sites on the CHC NTD directly interact with GTSE1. (A) ...
Published: 13 January 2020
Figure 1. Adaptor interaction sites on the CHC NTD directly interact with GTSE1. (A) Immunoblots of cell lysates (input) and IPs of GTSE1 and CHC in U2OS and GTSE1 knockout (GTSE1 KO ) cells. Immunoblots with anti-GTSE1 or anti-CHC antibody. (B) Immunoblot of cell lysates (input) and IPs of GT... More
Images
Clathrin recruits GTSE1 to the spindle. (A)  Immunofluorescence images of U...
Published: 13 January 2020
Figure 2. Clathrin recruits GTSE1 to the spindle. (A) Immunofluorescence images of U2OS cells stably expressing an RNAi-resistant GTSE1-GFP BAC transgene, stained with antibodies against GFP and α-tubulin. Cells were transfected with control (Ctrl) or CHC siRNA (66 h). All cells were concurrentl... More
Images
RNAi depletion efficiencies. (A)  Immunoblot on the cell lysate of U2OS cel...
Published: 13 January 2020
Figure S1. RNAi depletion efficiencies. (A) Immunoblot on the cell lysate of U2OS cells expressing an siRNA-resistant GTSE1-GFP BAC transgene and transfected with control (Ctrl) or CHC siRNA for 66 h. Cells were concomitantly transfected with GTSE1 siRNA to deplete endogenous GTSE1. Immunoblots ... More
Images
Multiple CBMs on GTSE1 and adaptor interaction sites on CHC are required fo...
Published: 13 January 2020
Figure 3. Multiple CBMs on GTSE1 and adaptor interaction sites on CHC are required for the GTSE1–CHC interaction. (A) Sequence alignment of GTSE1 C-terminus (aa 661–727 of human GTSE1). Putative clathrin-binding motifs are in green; divergent motifs are italicized. Mutations in the “5xLID” mutan... More
Images
CBMs on the GTSE1 C-terminus interact with adaptor binding sites on the CHC...
Published: 13 January 2020
Figure S2. CBMs on the GTSE1 C-terminus interact with adaptor binding sites on the CHC NTD. (A) GST-pulldown analysis of the interaction between GST-CHC(1–364) and GTSE1 fragments fused to a His-tag (GTSE1-His). Numbers indicate amino acids present in the fragments. Immunoblot is with anti-His a... More
Images
Sequence alignments of GTSE1 and TACC3. (A)  MUSCLE alignment of the N-term...
Published: 13 January 2020
Figure S3. Sequence alignments of GTSE1 and TACC3. (A) MUSCLE alignment of the N-terminus (amino acids 1–143 from human GTSE1) and C-terminus (amino acids 679–739 from human GTSE1) of potential GTSE1 homologues. Alignment was performed on full-length genes from the indicated organisms. A conserv... More