Issues

The authors identify the conserved unconventional class XV myosin, Myo15, an actin motor, as a novel regulator of presynaptic assembly and remodeling in Drosophila. They find Myo15 to be essential for presynaptic functional homeostatic plasticity and memory consolidation.

Kim et al. report on Arc, a large PDZ domain–containing cell membrane–associated protein that controls Drosophila salivary gland architecture. Arc facilitates apical membrane delivery of Crumbs; in turn, Crumbs limits accumulation and activity of non-muscle myosin II to regulate the number of salivary gland cells that internalize at a given time.

Morgan et al. demonstrate that pan-expansion microscopy is an exceptional tool for probing the molecular composition and structural plasticity of individual NPCs: they reveal LINC complex–dependent NPC diameter biases across the nuclear surface and changing nucleoporin positions in an ALS model.

This work reveals essential roles for TUBD1 in male germ cell development and male fertility, specifically in stabilizing the meiotic kinetochores during division and shaping the sperm head via the manchette. Our data suggest that TUBD1 interacts with katanins to achieve microtubule severing.

Neurons and muscles must maintain structural contact despite mechanical strain. Ermanoska et al. identify a presynaptic actomyosin core at Drosophila neuromuscular junctions that coordinates with integrin adhesion receptors to sense and respond to tension changes, preserving neuron–muscle mechanical continuity.

Narula and Wignall combine high-resolution imaging with auxin-inducible degradation to demonstrate that PLK-1 suppresses centrosome maturation and dampens microtubule polymerization in C. elegans oocytes. Since this essential kinase promotes centrosome maturation during mitosis, these findings reveal that PLK-1 plays a noncanonical role to promote the fidelity of the meiotic divisions.

Teixeira et al. identify the onconeural antigen CDR2 as an ER-localized adaptor for the microtubule-based motor cytoplasmic dynein-1 and show that CDR2 contributes to ER organization through its interaction with the integral ER membrane protein kinectin.

Pasolli, Meiring et al. describe tools to acutely relocate vimentin intermediate filaments by inducibly coupling them to microtubule motors. Rapid perinuclear clustering of vimentin had no strong effects on microtubules or actin but was accompanied by redistribution of mitochondria and endoplasmic reticulum sheets, and reduced cell stiffness.