Issues

Wethekam and Moore investigate how cells balance levels of α- and β-tubulin to supply αβ-heterodimers. They found that cells produce higher levels of α-tubulin than β-tubulin and require this excess to limit β-tubulin accumulation. β-tubulin accumulation causes microtubule loss and formation of dead-end tubulin assemblies.

Martins et al. show that all septins associated with actin stress fibers organize as octamer-based filaments that mediate actin-membrane anchoring. Depleting octamers or preventing septins from polymerizing leads to a partial loss of stress fibers and compromised cell mechanics.

Djannatian et al. show by ultrastructural analysis of mouse optic nerves and in vivo imaging of zebrafish spinal cords that developmental myelination is an error-prone process and microglia contribute to the removal of aberrant structures. This may be an important mechanism to generate properly functioning myelin.

Berman et al. examine the capping activity of the γ-TuRC. Using in vitro and cellular assays, they characterize how the γ-TuRC's nucleotide-independent capping activity regulates microtubule dynamics and how it contributes to microtubule formation during cell division.

Hennlein et al. show a novel role of the actin-bundler PLS3 that mediates correct surface translocation of the neurotrophin receptor TrkB and proper "cluster-like" formations of voltage-gated Ca²⁺ channels as processes that are indispensable for development and functional maintenance of motoneurons.

Neurite pruning, a critical neuronal circuit specification mechanism, often involves neurite abscission by unknown mechanisms. In Drosophila sensory neurons, pruning dendrites first become mechanically fragile, and severing occurs through morphogenetic tissue movements that exert mechanical forces on the destabilized dendrites, thus establishing mechanical force as a mechanism during neurite pruning.

A screen for mutants defective in dynein-mediated early endosome transport identified a kinesin-1 mutation that disrupts kinesin-1 autoinhibition. Kinesin-1 autoinhibition is not needed for driving dynein accumulation at microtubule plus ends, but it affects the initiation of dynein-mediated cargo transport.

Mund and Tschanz et al. reveal the three-dimensional shapes of clathrin coats during endocytosis, which partially preassemble and then bend progressively. They further introduce the novel Cooperative Curvature Model, which accurately describes the changes in shapes and dynamics during endocytic clathrin remodeling.

Vander Wende et al. show that Superoxide dismutase 1 (Sod1), an antioxidant whose aggregation is associated with Amyotrophic Lateral Sclerosis (ALS), is highly regulated during meiosis. This includes regulation of its synthesis, aggregation, and degradation, allowing cells to refresh their pool of this important protein as rejuvenated gametes are created.

Xue et al. develop a system to visualize and compare focal adhesion formation and dynamics during single-cell migration in animal models versus cell culture systems. They reveal fundamental differences in the regulation of key focal adhesion proteins.

Miller et al. identify the tomosyn homolog Sro7 as an allosteric activator of the exocyst resulting in specific biochemical changes to the complex. The outcomes of exocyst activation by Sro7 are distinct and insulated from exocyst activation by Rho/Cdc42 GTPases.

Beppler et al. show that therapeutic T cells engineered to express chimeric antigen receptors (CARs) use the same dynamic finger-like projections, called microvilli, that facilitate natural T cell receptor (TCR) antigen scanning, but with hyper-stabilized dynamics following receptor binding.

Rocha et al. show that C. elegans protein phosphatase 4 ensures robust outer kinetochore assembly prior to nuclear envelope breakdown, which is critical for timely and proper engagement of mitotic chromosomes with spindle microtubules.

We present a method probing the local membrane environment surrounding membrane proteins in the plasma membrane by linking a solvatochromic dye to the target, and provide evidence for lipid-based sorting. The insulin receptor environment changed upon insulin stimulation revealing dynamic local changes and distance dependence.

Lleti, Steyer, and Schieber et al. present an integrated software that automates correlative light and volume electron microscopy to perform high-resolution volumetric imaging of multiple cells.