How autophagy in neurons is regulated by synaptic activity is largely unknown. Kulkarni et al. show that synaptic activity decreases the motility and increases the degradative function of autophagic vacuoles within dendrites but not axons.
How EPH/EPHRIN signaling regulates the biophysical properties of cells to drive tissue organization is not well understood. Kindberg et al. find that EPH/EPHRIN signaling drives cellular organization by modulating cell contact strength by regulating actomyosin contractility at heterotypic contacts.
Wang et al. show that meiotic spindle assembly in Drosophila oocytes depends on the Borealin subunit of the chromosome passenger complex (CPC). Borealin does this by colocalizing with HP1, recruiting the CPC to the chromatin, and mediating movement of the CPC to the microtubules.
Zaman and Lombardo et al. characterize cells lacking all ERM proteins or their activating kinases. This reveals that active ERMs are local negative regulators of RhoA necessary to mediate the architecture of the apical domain.
Robertson et al. show that unlike T cells responding to conventional chemokines, T cells responding to the lipid chemoattractant S1P utilize a bleb-based mode of motility. This response involves myosin-induced increases in intracellular pressure and is directed by the cortical cytoskeletal proteins ezrin and moesin.
Correct mitotic entry requires inhibition of PP2A-B55 before nuclear envelope breakdown. This inhibition is mediated by Greatwall-phosphorylated Endosulfine. This works shows Endos and PP2A-B55 must function in the cytoplasm, which explains why nuclear Greatwall relocates to the cytoplasm in prophase.
Dello Stritto et al. provide evidence that DNA lesions in both germline mitotic and meiotic compartments are less capable of triggering apoptosis in the absence of topoisomerase 3. In topoisomerase 3 mutants, uncontrolled bloom helicase activity governs repair of defective recombination intermediates to evade apoptosis.
RNA:DNA hybrids are important intermediates in cellular processes. Smolka et al. establish controls to test the specificity of the S9.6 anti-RNA:DNA hybrid antibody in imaging approaches. They found that S9.6 immunofluorescence signal derives primarily from ribosomal RNA, not RNA:DNA hybrids.