Larocque et al. describe how tumor protein D52-like proteins associate with intracellular nanovesicles. These small transport vesicles are shown to be involved in recycling α5β1 integrins during cell migration and invasion.
Belicova et al. report previously unrecognized apical membrane extensions, forming a pattern reminiscent of the bulkheads of boats, that determine the anisotropic expansion of hepatocyte lumina. Loss of the bulkheads caused by Rab35 silencing leads to re-engineering of epithelial polarity and liver tissue architecture.
Frazer et al. use 4D imaging to reveal that while the intracellular pathways orchestrating killing within CTL are conserved, the rate of initiation along these pathways varies with TCR signal strength.
This work dissects the role of different KIF1A tail domains in motor activity and cargo binding. Based on the results, the authors propose a mechanism for the regulation of KIF1A cargo transport in hippocampal neurons.
This work identifies endoplasmic reticulum subdomains, where the sterol synthesis and export machineries are coupled to sustain endocytosis in yeast mother cells, but not in daughters, where endocytosis and plasma membrane loading with accessible sterols are linked to secretion.
Gilles et al. reveal that the phospholipase NOT-LIKE-DAD localizes on the endo-plasma membrane that surrounds the sperm cells in a pollen grain. This endo-PM has a PI(4,5)P2 lipid signature, and NOT-LIKE-DAD is addressed by lipid anchoring and electrostatic interactions. After pollen tube burst, the endo-PM locates within the embryo sac and helps to deliver the sperm cells.
Molenaar et al. show that seipin does not play a role in the biogenesis of lipid droplets composed mostly or only of the neutral lipid retinyl ester, even in cells that produce triacylglycerols.
Fast-growing microtubules are associated with large protective GTP-caps, raising the question of how cells achieve simultaneously fast and highly dynamic microtubule growth. Farmer et al. show that polymerase XMAP215 perturbs the growing microtubule end to promote microtubule catastrophe, despite simultaneously accelerating the microtubule growth rate.