Stable eukaryotic flagella can maintain their length, and many protists grow new flagella while maintaining existing flagella. Mechanisms regulating this are not well understood. Atkins et al. show that CEP164C is important for this regulation, and these findings provide key novel insights into this process.
Twinfilin bypasses assembly conditions and actin filament aging to drive barbed end depolymerization
Shekhar and Hoeprich et al. show that Twinfilin bypasses the normal requirement for actin filament aging (Pi release) and drives barbed end depolymerization of newly formed filaments. This occurs even under assembly-promoting conditions, where actin monomer levels are far above the critical concentration.
Wakana et al. describe a new role for SCAP, a key regulatory protein in cholesterol metabolism, in promoting Golgi PI4P turnover and the biogenesis of the TGN-derived transport carriers CARTS via interaction with the cholesterol/PI4P exchange machinery at ER–Golgi membrane contact sites.
Hedgehog (Hh) is an essential signaling pathway for embryonic development and adulthood. Here, we show that noncanonical Hh induces the formation of primary cilia, the critical signaling hubs whose absence leads to a variety of severe disorders, via two distinct, critical pathways.
In mechanoreceptor cells, specialized cytoskeletons are formed at the site of mechanotransduction. Sun et al. reconstruct the microtubule-based cytoskeleton in fly campaniform mechanosensory cilia using electron microscopic tomography and establish dual functions for katanin p60-like 1 in shaping the cytoskeleton.