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Cover Image
Cover Image
On the cover
An electron tomography-based 3D reconstruction of the C. elegans ciliary transition zone shows the central cylinder (brown) and Y-links (red) connecting axonemal microtubules (yellow) to the ciliary membrane (green). Schouteden et al. demonstrate that complete disruption of the transition zone only mildly affects axoneme assembly, but strongly impairs the extension of dendrites by retrograde migration, revealing an unexpected function for this ciliary substructure in cell-matrix adhesion.
Image © 2015 Schouteden et al.
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In This Issue
In Focus
People & Ideas
Sally Horne-Badovinac: Taking a spin around morphogenesis
Horne-Badovinac tracks how coordinated cellular movements mold tissues.
Feature
Review
Report
CENP-C is a blueprint for constitutive centromere–associated network assembly within human kinetochores
CENP-C promotes kinetochore targeting of other constitutive centromere–associated network (CCAN) subunits by directly interacting with the four-subunit CCAN subcomplex CENP-HIKM and spatially organizing the localization of all other CCAN subunits downstream of CENP-A.
Control of the pericentrosomal H2O2 level by peroxiredoxin I is critical for mitotic progression
Reversible oxidative inactivation of centrosome-bound protein phosphatases such as Cdc14B by H2O2 is likely responsible for the inhibition of Cdk1-opposing phosphatases during early mitosis, which prevents premature degradation of mitotic activators.
The ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in C. elegans
C. elegans transition zone structures are dispensable for axoneme assembly but are required for cell–matrix interactions during neurite extension, revealing an unexpected role for the transition zone in cell adhesion.
Article
Mio depletion links mTOR regulation to Aurora A and Plk1 activation at mitotic centrosomes
Mio, a conserved member of the SEACAT/GATOR2 complex, is required for concentration of active Plk1 and Aurora kinases at spindle poles and for subsequent spindle formation and chromosome segregation.
p53 protects against genome instability following centriole duplication failure
Reversible depletion of centrioles uncovers a p53-dependent pathway that protects against genome instability following centriole duplication failure.
Interphase centrosome organization by the PLP-Cnn scaffold is required for centrosome function
Cnn and PLP directly interact at two defined sites to coordinate the cell cycle–dependent rearrangement and scaffolding activity of the centrosome to permit normal centrosome organization, cell division, and embryonic viability.
A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit
The fission yeast cytoskeleton is completely reorganized upon quiescence entry: the microtubule (MT) cytoskeleton is drastically reshaped as quiescent cells assemble a large spindle pole body–associated MT bundle composed of stable antiparallel MTs.
VAMP8-dependent fusion of recycling endosomes with the plasma membrane facilitates T lymphocyte cytotoxicity
VAMP8 is associated with the recycling endosome compartment rather than with cytotoxic granules and is required for a fusion step between recycling endosomes and the plasma membrane that brings syntaxin-11 to the immune synapse for cytotoxic granule exocytosis.
Schwann cell autophagy, myelinophagy, initiates myelin clearance from injured nerves
Schwann cells degrade myelin after injury by a novel form of selective autophagy, myelinophagy, which is positively regulated by the JNK/c-Jun pathway and is defective in the injured central nervous system.
The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium
Rpgrip1l regulates proteasomal activity at the basal body via Psmd2 and thereby controls ciliary signaling.
