-
Cover Image
Cover Image
On the cover
In a budding yeast cell overexpressing the nuclear membrane protein Kar1, the spindle pole body bridge—a structure that promotes the organelle’s duplication—is abnormally extended. Seybold et al. reveal that Kar1 anchors the bridge to the nuclear envelope and, together with Cdc31, stabilizes its other cytoplasmic component, Sfi1.
Image © 2015 Seybold et al.
See page 843. - PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
In Focus
GMFβ prunes actin branches
Study reveals that turnover of lamellipodial actin networks helps fibroblasts follow adhesive guidance cues.
In This Issue
People & Ideas
Elena Gracheva: Ion channels run hot and cold
Gracheva studies the molecular basis and evolutionary origins of hibernation.
From the Archive
The first buds of Cdc42
In 1990, John Pringle and colleagues identified the small GTPase and demonstrated its role in yeast cell polarization.
Review
Report
A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance
Studying CENP-A nucleosome assembly in a cell-free system defines the role of existing CENP-A nucleosomes in centromere maintenance.
GMFβ controls branched actin content and lamellipodial retraction in fibroblasts
The primary activity of GMFβ in vivo is actin branch disassembly (and not inhibition of Arp2/3 activation), and this activity plays an important role in lamellipodial dynamics and directional migration toward ECM cues.
Article
Centromere protein F includes two sites that couple efficiently to depolymerizing microtubules
Both N- and C-terminal microtubule (MT)-binding domains of CENP-F can follow depolymerizing MT ends while bearing a significant load, and the N-terminal domain prefers binding to curled oligomers of tubulin relative to MT walls by approximately fivefold, suggesting that CENP-F may play a role in the firm bonds that form between kinetochores and the flared plus ends of dynamic MTs.
Pheromone-encoding mRNA is transported to the yeast mating projection by specific RNP granules
In response to mating pheromone, the yeast MFA2 mRNA is transported to the tip of the mating projection as an RNP granule and translated; integrity of the granules is required for normal mRNA transport and for the mating process.
Kar1 binding to Sfi1 C-terminal regions anchors the SPB bridge to the nuclear envelope
Kar1 interacts with the C-terminal (CT) region of Sfi1 to tether the yeast centrosome’s bridge to the nuclear envelope; Sfi1-CT and C-terminal Cdc31-binding regions form the antiparallel Sfi1 overlap in the bridge.
Binding of STIL to Plk4 activates kinase activity to promote centriole assembly
Binding of STIL activates Plk4, and the subsequent phosphorylation of STIL by Plk4 primes the binding of STIL to SAS6 to promote centriole assembly.
Calcium release through P2X4 activates calmodulin to promote endolysosomal membrane fusion
P2X4 and calmodulin form a signaling complex in late endosomes and lysosomes that promotes fusion and vacuolation in a Ca2+-dependent fashion.
Tools
Kinase-interacting substrate screening is a novel method to identify kinase substrates
A novel method called kinase-interacting substrate screening based on affinity beads coated with the kinase of interest identifies phosphorylation sites for Rho-kinase and others, which reveals that Rho-kinase substrate Scrib plays a crucial role in the regulation of subcellular contractility by assembling with Rho-kinase and Shroom2.
