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Cover Image
Cover Image
On the cover
Shen et al. describe how the ESCRT-III subunit Vps32/CHMP4B assembles into spiral-shaped polymers. Individual spiral filaments in a cryo-electron micrograph are highlighted in a variety of colors.
Image © 2014 Shen et al.
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In This Issue
In Focus
Topoisomerase II has to work late
Chromatid-untangling enzyme takes longer than expected to complete job.
People & Ideas
Margaret Robinson: Vesicles wear fancy coats
Robinson studies the function and evolution of coated vesicle adaptor proteins.
Review
Report
Dlg1 controls planar spindle orientation in the neuroepithelium through direct interaction with LGN
Dlg1 recruits LGN to the cortex of cells in the chick neuroepithelium and may provide instructive cues that drive planar spindle orientation.
Article
Chromosome length and perinuclear attachment constrain resolution of DNA intertwines
Independent of the presence of rDNA repeats, topological constraints imposed by chromosome length and perinuclear attachment determine the efficiency with which sister chromatid intertwines are resolved by topoisomerase II and dynamic microtubules during anaphase.
Expression of HSF2 decreases in mitosis to enable stress-inducible transcription and cell survival
In spite of global transcriptional inhibition, a decrease in HSF2 expression during mitosis allows for heat shock protein expression and protects cells against proteotoxicity.
Concentration of Sec12 at ER exit sites via interaction with cTAGE5 is required for collagen export
By interacting with the collagen cargo receptor component cTAGE5, Sec12 concentrates at ER exit sites and generates the high levels of GTP-bound Sar1 necessary for export of collagen to the Golgi.
Structural analysis and modeling reveals new mechanisms governing ESCRT-III spiral filament assembly
Cryo-EM and molecular dynamics simulations reveal unexpected flexibility in individual monomers and a stable interface between monomers in the spiral filaments formed by the ESCRT-III subunit Vps32/CHMP4B.
Disease mutations in desmoplakin inhibit Cx43 membrane targeting mediated by desmoplakin–EB1 interactions
An interaction with the microtubule-binding protein EB1 enables the desmosomal protein desmoplakin to modify microtubule organization and dynamics near sites of cell–cell contact, subsequently regulating membrane localization of gap junction–forming connexins.
Activation of RhoA-ROCK-BMP signaling reprograms adult human corneal endothelial cells
Activation of RhoA-ROCK-BMP signaling reprograms adult human corneal endothelial cells into neural crest–like progenitors, which effectively form corneal endothelial monolayers that may eliminate the need for corneal transplantation.
