On the cover
Kuriyama et al. reveal that the phospholipid lysophosphatidic acid promotes the collective migration of Xenopus neural crest cells by downregulating the surface expression of N-cadherin, granting the cells fluid-like properties that enhance their movement through embryonic tissues. Neural crest cell membranes are labeled yellow, protrusions are labeled red, and nuclei are labeled blue.
Image © 2014 Kuriyama et al.
See page 113.
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In This Issue
Defining the kinetochore’s rules of engagement
Quantitative analyses and computer modeling reveal how a “molecular lawn” fine-tunes the interactions between kinetochores and microtubules.
People & Ideas
Sally Kornbluth: Nature’s incredible contraptions
Kornbluth’s laboratory studies cell cycle and apoptosis in cancer cells and Xenopus egg extracts.
Bud3 activates Cdc42 to establish a proper growth site in budding yeast
Biphasic activation of Cdc42 by Bud3 and then Cdc24 during G1 of the yeast cell cycle is necessary for assembly of a proper bud site.
DNA breaks and chromosomal aberrations arise when replication meets base excision repair
DNA double-strand breaks and chromosomal aberrations after treatment with N-alkylating agents likely arise as a result of replication fork collision with single-strand breaks generated during base excision repair.
Accurate phosphoregulation of kinetochore–microtubule affinity requires unconstrained molecular interactions
Accurate regulation of kinetochore–microtubule affinity is driven by incremental phosphorylation of an NDC80 molecular “lawn,” in which NDC80–microtubule bonds reorganize dynamically in response to the number and stability of microtubule attachments.
Pom1 regulates the assembly of Cdr2–Mid1 cortical nodes for robust spatial control of cytokinesis
Pom1 regulation of Cdr2 membrane association and interaction with Mid1 prevents Cdr2 assembly into stable nodes in the cell tip region, which ensures proper positioning of cytokinetic ring precursors and accurate division plane positioning in fission yeast.
Phosphatidic acid phospholipase A1 mediates ER–Golgi transit of a family of G protein–coupled receptors
Cytosolic phosphatidic acid phospholipase A1 interacts with COPII protein family members and is required for the anterograde trafficking of GPCRs.
Rac1 and Aurora A regulate MCAK to polarize microtubule growth in migrating endothelial cells
A Rac1–Aurora A–MCAK signaling pathway mediates endothelial cell polarization and directional migration by promoting regional differences in microtubule dynamics in the leading and trailing cell edges.
In vivo collective cell migration requires an LPAR2-dependent increase in tissue fluidity
Neural crest epithelial–mesenchymal transition (EMT) and collective cell migration rely on a solid-to-liquid-like transition triggered by internalization of N-cadherin downstream of lysophosphatidic acid receptor 2.
C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization
The scaffold protein ANI-2 promotes germ cell syncytial organization and compensates for the mechanical stress associated with oogenesis by conferring stability and elasticity to germ cell intercellular bridges.