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Cover Image
Cover Image
On the cover
Structured illumination microscopy shows an NIH 3T3 cell spread on a composite pattern of fibronectin (blue) and vitronectin (black). The cell is transfected to express a mutant β3 integrin (green) that binds talin with high affinity but is unable to support cell spreading on vitronectin. The signaling adaptor paxillin (red) is recruited to endogenous β1 integrin–based adhesions bound to fibronectin but is absent from vitronectin-associated, β3 integrin–based contacts.
Image © 2014 Pinon et al.
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In This Issue
In Focus
Not all vinculins are created equal
Cells modify force-transmitting protein for use in adherens junctions.
People & Ideas
Alex Mogilner: Math illuminates biology
Mogilner uses modeling to probe and explain the behavior of cells and their cytoskeleton.
Review
Report
PINK1 phosphorylates ubiquitin to activate Parkin E3 ubiquitin ligase activity
PINK1 phosphorylates ubiquitin, which then binds to Parkin and activates its E3 ligase activity, leading to induction of selective autophagy of damaged mitochondria.
Contractile forces regulate cell division in three-dimensional environments
Live-cell imaging, combined with mapping of 3D matrix displacements, identifies sites at which cells apply contractile forces to the matrix and reveals roles for physical forces in cell division.
Article
PKA-dependent dynein switching from lysosomes to adenovirus: A novel form of host–virus competition
PKA-mediated phosphorylation of a specific residue in the dynein light intermediate chain 1 releases the motor protein from lysosomes and late endosomes while activating its recruitment to adenovirus capsids.
Mitochondrial fusion is frequent in skeletal muscle and supports excitation–contraction coupling
Mitochondrial fusion is frequent in skeletal muscle, and its disruption jeopardizes excitation–contraction coupling and may contribute to the pathology of myopathies.
ProSAP1 and membrane nanodomain-associated syndapin I promote postsynapse formation and function
ProSAP1/Shank2 and syndapin I–enriched membrane nanodomains are important spatial cues and organizing platforms that shape dendritic membranes into synaptic compartments.
A novel Netrin-1–sensitive mechanism promotes local SNARE-mediated exocytosis during axon branching
Localized plasma membrane expansion during axon branching mediated by Netrin-1 occurs via TRIM9-dependent regulation of SNARE-mediated vesicle fusion.
BARP suppresses voltage-gated calcium channel activity and Ca2+-evoked exocytosis
The integral membrane glycoprotein BARP associates with voltage-gated calcium channel Cavβ subunits, disrupting their association with Cavα1 subunits and suppressing overall channel activity.
Vinculin phosphorylation differentially regulates mechanotransduction at cell–cell and cell–matrix adhesions
Vinculin phosphorylation on residue Y822 is necessary for cell stiffening in response to tension on cadherins but not integrins.
Talin-bound NPLY motif recruits integrin-signaling adapters to regulate cell spreading and mechanosensing
β3 integrin residue Y747 is required for cell spreading and paxillin adapter recruitment to substrate-bound integrins in response to substrate stiffness.
