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Cover Image
Cover Image
On the cover
In primary mouse keratinocytes, microtubules (green) decorated with the plus end-tracking protein CLASP2 (red) are targeted to intercellular contacts mediated by E-cadherin (blue). Shahbazi et al. demonstrate that CLASP2 interacts with adherens junctions via the cadherin-binding protein p120-catenin, controlling both microtubule dynamics and cell-cell adhesion.
Image © 2013 Shahbazi et al.
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In This Issue
In Focus
Arp2/3-deficient cells share their problems
Loss of the actin-nucleating complex activates a stress response that nonautonomously inhibits chemotaxis.
People & Ideas
Arnaud Echard: Adieu, ma soeur
Echard studies membrane trafficking and the cytoskeleton, with a focus on cytokinesis.
Review
Report
Spindle assembly checkpoint robustness requires Tpr-mediated regulation of Mad1/Mad2 proteostasis
Tpr is a kinetochore-independent, rate-limiting factor required to mount and sustain a robust spindle assembly checkpoint response by stabilizing Mad1 and Mad2 before mitosis.
Novel septin 9 repeat motifs altered in neuralgic amyotrophy bind and bundle microtubules
Novel septin 9 repeat motifs interact with the acidic C-terminal tails of β-tubulin to promote microtubule bundling and asymmetric neurite growth.
Loss of Arp2/3 induces an NF-κB–dependent, nonautonomous effect on chemotactic signaling
A decrease in Arp2/3 levels results in an NF-κB–dependent increase in the expression of several secreted factors, resulting in nonautonomous effects on chemotaxis.
Autophagy and metacaspase determine the mode of cell death in plants
Metacaspase-dependent autophagy in plants promotes cell disassembly during vacuolar cell death and inhibits necrosis.
Article
Higher-order unfolding of satellite heterochromatin is a consistent and early event in cell senescence
Higher-order unfolding of peri/centromeric satellite DNA is a consistent and early event in senescence of cultured normal human and mouse cells, progeria cells, and a senescent tumor.
Arrayed BUB recruitment modules in the kinetochore scaffold KNL1 promote accurate chromosome segregation
The kinetochore scaffold KNL1 contains an extensive array of short linear sequence modules that each independently can localize BUB1 but that together provide enhanced efficiency of chromosome segregation.
KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity
KNL1 is essential for efficient kinetochore–microtubule attachment dynamics during mitosis, in part through promotion of Bub1-mediated targeting of Aurora B to the kinetochore.
In vitro reconstitution of an mRNA-transport complex reveals mechanisms of assembly and motor activation
The budding yeast SHE mRNA-transport complex dimerizes to activate processive RNA transport, irrespective of the presence of RNA cargo, and multimerizes upon binding RNAs with multiple localization elements.
Acyl-CoA synthetase 3 promotes lipid droplet biogenesis in ER microdomains
Acyl-CoA synthetase 3 is recruited early to lipid droplet assembly sites on the ER, where it is required for efficient lipid droplet nucleation and lipid storage.
JAK tyrosine kinases promote hierarchical activation of Rho and Rap modules of integrin activation
JAK tyrosine kinases promote integrin activation and integrin-meditated lymphocyte trafficking by bridging chemokine receptors to activation of Rho and Rap.
The N terminus of SKAP55 enables T cell adhesion to TCR and integrin ligands via distinct mechanisms
In addition to promoting integrin activation at the immunological synapse, SKAP55 links T cell antigen receptor-associated signaling molecules to the cytoskeleton and promotes integrin-independent adhesion via the T cell receptor.
CLASP2 interacts with p120-catenin and governs microtubule dynamics at adherens junctions
The microtubule plus end–binding protein CLASP2 localizes to adherens junctions via direct interaction with p120-catenin and is required for adherens junction stability.
Endosomal WASH and exocyst complexes control exocytosis of MT1-MMP at invadopodia
WASH and exocyst promote pericellular matrix degradation and tumor cell invasion by enabling localized exocytosis of MT1-MMP from late endosomes.
Filamentous morphology of bacteria delays the timing of phagosome morphogenesis in macrophages
Uptake of bacterial filaments by macrophages is characterized by a prolonged phagocytic cup stage and diminished microbicidal activity during phagosome maturation.
