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Cover Image
Cover Image
On the cover
Progerin (blue), a mutant form of lamin A found in patients with Hutchinson-Gilford Progeria syndrome, inhibits the nuclear import of pyruvate kinase-SV40NLS (red) but permits the import of streptavidin-SV40NLS (green). In the absence of Progerin, however, both reporter proteins are nuclear. Snow et al. reveal that larger protein cargoes, such as pyruvate kinase-SV40NLS, are more sensitive to the altered Ran GTPase distribution caused by Progerin expression.
Image © 2013 Snow et al.
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In This Issue
In Focus
People & Ideas
Review
Report
Gain-of-function mutations of PPM1D/Wip1 impair the p53-dependent G1 checkpoint
Mutations in PPM1D/Wip1 phosphatase impair the DNA damage-induced checkpoint and may predispose cells to tumorigenesis.
The asymmetric segregation of damaged proteins is stem cell–type dependent
Asymmetric segregation of damaged proteins is detectable in three different adult tissue stem cells in Drosophila melanogaster but does not always favor segregation away from the stem cell.
Autophagosomal Syntaxin17-dependent lysosomal degradation maintains neuronal function in Drosophila
Lysosomal degradation and recycling of sequestered autophagosome content is crucial to maintain proper functioning of the fly nervous system.
Article
Defective nuclear import of Tpr in Progeria reflects the Ran sensitivity of large cargo transport
Nuclear transport of large protein cargoes such as Tpr is more sensitive to the alteration of the ratio of nuclear to cytoplasmic Ran that occurs in Progeria.
The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A
UBXN-2, a substrate adaptor of the AAA ATPase CDC-48/p97, is required to coordinate centrosome maturation timing with mitosis.
Dynamic bonds and polar ejection force distribution explain kinetochore oscillations in PtK1 cells
A computational model of kinetochore dynamics suggests that differences in the distribution of polar ejection forces at the periphery and in the middle of PtK1 cell spindles underlie the observed position-dependence of metaphase chromosome behavior.
Ligand-induced activation of a formin–NPF pair leads to collaborative actin nucleation
Bil1 binds to the regulatory sequence of Bud6, unmasking its ability to stimulate the activity of the formin Bni1 and promote actin nucleation.
p53-dependent release of Alarmin HMGB1 is a central mediator of senescent phenotypes
In addition to allowing immune cells to signal tissue damage, HMBG1 is secreted by senescent cells to initiate inflammatory cytokine secretion.
Tools
A light-triggered protein secretion system
Secreted proteins fused to the plant photoreceptor protein UVR8 are conditionally sequestered in the ER until a pulse of light triggers trafficking through the secretory pathway, allowing precise control of forward secretory trafficking.
