On the cover
Progerin (blue), a mutant form of lamin A found in patients with Hutchinson-Gilford Progeria syndrome, inhibits the nuclear import of pyruvate kinase-SV40NLS (red) but permits the import of streptavidin-SV40NLS (green). In the absence of Progerin, however, both reporter proteins are nuclear. Snow et al. reveal that larger protein cargoes, such as pyruvate kinase-SV40NLS, are more sensitive to the altered Ran GTPase distribution caused by Progerin expression.
Image © 2013 Snow et al.
See page 541.
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The cell biology of disease
Mutations in PPM1D/Wip1 phosphatase impair the DNA damage-induced checkpoint and may predispose cells to tumorigenesis.
Asymmetric segregation of damaged proteins is detectable in three different adult tissue stem cells in Drosophila melanogaster but does not always favor segregation away from the stem cell.
Lysosomal degradation and recycling of sequestered autophagosome content is crucial to maintain proper functioning of the fly nervous system.
Nuclear transport of large protein cargoes such as Tpr is more sensitive to the alteration of the ratio of nuclear to cytoplasmic Ran that occurs in Progeria.
The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A
UBXN-2, a substrate adaptor of the AAA ATPase CDC-48/p97, is required to coordinate centrosome maturation timing with mitosis.
A computational model of kinetochore dynamics suggests that differences in the distribution of polar ejection forces at the periphery and in the middle of PtK1 cell spindles underlie the observed position-dependence of metaphase chromosome behavior.
Bil1 binds to the regulatory sequence of Bud6, unmasking its ability to stimulate the activity of the formin Bni1 and promote actin nucleation.
In addition to allowing immune cells to signal tissue damage, HMBG1 is secreted by senescent cells to initiate inflammatory cytokine secretion.
Secreted proteins fused to the plant photoreceptor protein UVR8 are conditionally sequestered in the ER until a pulse of light triggers trafficking through the secretory pathway, allowing precise control of forward secretory trafficking.