-
Cover Image
Cover Image
On the cover
Lechtreck et al. reveal that the BBSome complex exports signaling proteins out of cilia. Though phospholipase D (PLD) accumulates in the cilia of Chlamydomonas cells lacking the BBSome subunit BBS4, mating these mutants with wild-type algae generates quadraciliated zygotes (pictured), in which the BBSome's function is restored, leading to the rapid export of PLD (red) from cilia (green) to the cell body.
Image © 2013 Lechtreck et al.
See page 249. - PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
In This Issue
In Focus
Setting an immuno-TRAP for PML nuclear bodies
Researchers describe technique to identify genomic loci that interact with specific nuclear structures.
People & Ideas
Margaret Gardel: At the interface of physics and biology
Gardel studies the biophysical properties of cells and cellular force generation.
Review
Report
Xenopus oocyte meiosis lacks spindle assembly checkpoint control
In contrast to the situation in mammalian oocytes, the metaphase-to-anaphase transition in frog oocytes is not regulated by a spindle assembly checkpoint.
Article
Systematic dissection of dynein regulators in mitosis
A comprehensive survey of the roles of dynein subunits and adaptors in mitosis reveals that dynein forms distinct complexes requiring specific recruiters and activators to promote orderly progression through mitosis.
A TPR domain–containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B
The kinetochore localization of MPS1 is necessary for mitotic checkpoint activity and requires the microtubule-binding domain of HEC1 and the Aurora B–dependent regulation of the TPR domain.
Microtubule-dependent endosomal sorting of clathrin-independent cargo by Hook1
Hook1, a microtubule and cargo tethering protein, is important for the sorting of clathrin-independent cargoes away from EEA1+ endosomes and promotes their recycling.
Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase
The BBSome, a regulator of ciliary membrane protein composition, is required only for the export phase of a process that continuously cycles phospholipase D through cilia.
The MIA complex is a conserved and novel dynein regulator essential for normal ciliary motility
The MIA complex, composed of FAP100 and FAP73, interacts with I1 dynein components and is required for normal ciliary beat frequency.
Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia
Hypoxia increases the levels of ADAM12 in a Notch-dependent manner, leading to increased ectodomain shedding of HB-EGF and subsequent promotion of invadopodia formation.
Ablation of the mTORC2 component rictor in brain or Purkinje cells affects size and neuron morphology
The mTOR complex 2 (mTORC2) is essential in the central nervous system for normal neuronal structure and function, potentially through effects on PKC signaling and independent of the related mTOR complex 1 (mTORC1).
Smooth muscle fascicular reorientation is required for esophageal morphogenesis and dependent on Cdo
Cdo-deficient mice have defects in smooth muscle fascicular reorientation during esophageal morphogenesis, resulting in structural and functional defects including an aberrantly proximal skeletal–smooth muscle boundary and achalasia.
Tools
Identifying gene locus associations with promyelocytic leukemia nuclear bodies using immuno-TRAP
A new immuno-TRAP technique overcomes limitations of spatial resolution and selection bias to identify gene locus associations with a nuclear subcompartment such as promyelocytic leukemia nuclear bodies.
Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox
Fluorescent lifetime imaging of an ER-tuned redox-responsive probe revealed an unanticipated stability of ER thiol redox to fluctuations in unfolded protein load, in contrast with sensitivity to lumenal calcium.
